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<p>Mungbean (<i>Vigna radiata</i> L. Wilczek) is a vital grain legume whose productivity is often constrained by limited phosphorus availability. Phosphorus deficiency hampers plant growth and alters root morphology, typically increasing the root-to-shoot ratio. Enhancing phosphorus-use efficiency (PUE) is therefore essential for improving mungbean performance under nutrient-limited conditions. To explore the genetic basis of PUE, transcriptome sequencing was performed on leaves, stems, and roots of two contrasting genotypes: Pusa 1333 (phosphorus-efficient) and PS 16 (phosphorus-inefficient). RNA-seq analysis revealed 833 upregulated and 1081 downregulated genes across tissues. In roots, 137 genes were upregulated and 477 downregulated; in stems, 365 were upregulated and 294 downregulated; and in leaves, 331 were upregulated and 310 downregulated. Gene Ontology (GO) classification indicated enrichment in biological processes related to growth, metabolism, and cellular organization. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that differentially expressed genes (DEGs) were predominantly associated with metabolic activity and secondary metabolite biosynthesis. Notably, eight DEGs were consistently expressed across all three tissues. Among these, <i>Vradi05g03810</i> (polygalacturonase) interacted with cell wall–related genes, suggesting a role in modulating cell wall dynamics under phosphorus stress. <i>Vradi05g03870</i> (RD22-like glycosyl transferase) interacted with stress-responsive proteins, indicating involvement in dehydration tolerance. These findings provide novel insights into the molecular regulation of PUE in mungbean and highlight candidate genes for breeding programs aimed at improving phosphorus efficiency and resilience under nutrient-limited conditions.</p>

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Preliminary Investigation of Phosphorus-Use Efficiency in Mungbean (Vigna radiata): A Comparative RNA-Seq Study

  • 期刊论文

  • 发表日期：

    2026年6月17日

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  • Deepali Kothari,
  • Muraleedhar S. Aski,
  • S. Premakumar,
  • Bidwan Rath,
  • Nirmala Pargaien,
  • Lalit Mohan Tewari,
  • Shouvik Das,
  • Gyan Prakash Mishra,
  • Gyanendra Singh,
  • Prachi Shripatrao Yadav,
  • Ya-Ping Lin,
  • Roland Schafleitner,
  • Ramakrishnan M. Nair,
  • Harsh Kumar Dikshit

摘要

Mungbean (Vigna radiata L. Wilczek) is a vital grain legume whose productivity is often constrained by limited phosphorus availability. Phosphorus deficiency hampers plant growth and alters root morphology, typically increasing the root-to-shoot ratio. Enhancing phosphorus-use efficiency (PUE) is therefore essential for improving mungbean performance under nutrient-limited conditions. To explore the genetic basis of PUE, transcriptome sequencing was performed on leaves, stems, and roots of two contrasting genotypes: Pusa 1333 (phosphorus-efficient) and PS 16 (phosphorus-inefficient). RNA-seq analysis revealed 833 upregulated and 1081 downregulated genes across tissues. In roots, 137 genes were upregulated and 477 downregulated; in stems, 365 were upregulated and 294 downregulated; and in leaves, 331 were upregulated and 310 downregulated. Gene Ontology (GO) classification indicated enrichment in biological processes related to growth, metabolism, and cellular organization. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that differentially expressed genes (DEGs) were predominantly associated with metabolic activity and secondary metabolite biosynthesis. Notably, eight DEGs were consistently expressed across all three tissues. Among these, Vradi05g03810 (polygalacturonase) interacted with cell wall–related genes, suggesting a role in modulating cell wall dynamics under phosphorus stress. Vradi05g03870 (RD22-like glycosyl transferase) interacted with stress-responsive proteins, indicating involvement in dehydration tolerance. These findings provide novel insights into the molecular regulation of PUE in mungbean and highlight candidate genes for breeding programs aimed at improving phosphorus efficiency and resilience under nutrient-limited conditions.