Scholarlink

<p>Phytochemical composition and biological activities of methanolic extracts obtained from the flowers, leaves, and branches of chasteberry (<i>Vitex agnus-castus</i>) cultivated in Aydin, Türkiye. The extracts contained substantial levels of bioactive compounds, with Total Phenolic Content (TPC) reaching up to 672.7&#xa0;mg GAE/g in purple Koçarli flower extracts and Total Flavonoid Content (TFC) up to 25.1&#xa0;mg QE/g in leaves. High-performance liquid chromatography (HPLC) profiling confirmed marked chemical variability between plant parts, with flowers accumulating higher amounts of hesperidin, whereas leaves were richer in vanillic acid. Functionally, the extracts exhibited strong antioxidant capacity (CUPRAC values up to 2340.5&#xa0;µmol TE/g), as well as notable antifungal activity against Candida albicans. The fractions, particularly APF and KMF, demonstrated time- and dose-dependent reduction in cell viability of TPC-1 thyroid cancer cells as measured by MTT assay, with APF exhibiting the strongest cytotoxic effect (IC50 = 31.25&#xa0;μg/mL at 48&#xa0;h). Gas chromatography-mass spectrometry (GC–MS) analysis of the methanolic extracts revealed that oleic acid and its isomers (9‑octadecenoic acid (E) and (Z)) were the predominant constituents across all plant organs, with branches showing especially high levels (66.68%–75.76% of total ion current), while flowers and leaves contained lower proportions (≈20%–24%) and a more chemically diverse mixture of fatty acids, phenolic compounds and other organic metabolites. Therefore <i>V. agnus‑castus</i> containing more diverse phenolic and volatile constituents, together contributed to the observed in vitro antioxidant, antimicrobial and cytotoxic activities.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Fingerprinting and biofunctional evaluation of Vitex agnus-castus: in vitro antioxidant, antimicrobial activities and cytotoxicity against thyroid cancer cells

  • 期刊论文

  • 发表日期：

    2026年7月4日

查看全文
  • Elif Apaydın

摘要

Phytochemical composition and biological activities of methanolic extracts obtained from the flowers, leaves, and branches of chasteberry (Vitex agnus-castus) cultivated in Aydin, Türkiye. The extracts contained substantial levels of bioactive compounds, with Total Phenolic Content (TPC) reaching up to 672.7 mg GAE/g in purple Koçarli flower extracts and Total Flavonoid Content (TFC) up to 25.1 mg QE/g in leaves. High-performance liquid chromatography (HPLC) profiling confirmed marked chemical variability between plant parts, with flowers accumulating higher amounts of hesperidin, whereas leaves were richer in vanillic acid. Functionally, the extracts exhibited strong antioxidant capacity (CUPRAC values up to 2340.5 µmol TE/g), as well as notable antifungal activity against Candida albicans. The fractions, particularly APF and KMF, demonstrated time- and dose-dependent reduction in cell viability of TPC-1 thyroid cancer cells as measured by MTT assay, with APF exhibiting the strongest cytotoxic effect (IC50 = 31.25 μg/mL at 48 h). Gas chromatography-mass spectrometry (GC–MS) analysis of the methanolic extracts revealed that oleic acid and its isomers (9‑octadecenoic acid (E) and (Z)) were the predominant constituents across all plant organs, with branches showing especially high levels (66.68%–75.76% of total ion current), while flowers and leaves contained lower proportions (≈20%–24%) and a more chemically diverse mixture of fatty acids, phenolic compounds and other organic metabolites. Therefore V. agnus‑castus containing more diverse phenolic and volatile constituents, together contributed to the observed in vitro antioxidant, antimicrobial and cytotoxic activities.