Scholarlink

<p><i>Colletia paradoxa</i> is an endangered species with ornamental and medicinal potential. This study aimed to evaluate the effect of the culture medium and concentrations of plant growth regulators (PGRs) to establish an efficient protocol for plants production through <i>in vitro</i> cultivation. Nodal segments were cultured in MS, MS/2, MSM/2 and WPM media to determine the best culture medium. During the shoot development phase, the MS/2 medium was supplemented with 2.5, 5.0, and 10.0&#xa0;µM of 6-benzylaminopurine (BA) and a control. The addition of 5.0&#xa0;µM of cytokinins kinetin (KIN), zeatin (ZEA), and 2-isopentenyladenine (2IP) was tested. Rooting was assessed by soaking the base of the shoots in indole-3-butyric acid (IBA) solutions (2.5&#xa0;mM, 5.0&#xa0;mM, and 10.0&#xa0;mM) for five minutes before transferring them to MS/2 medium. The best shoot development and nodal culture occurred with 5.0&#xa0;µM BA, resulting in 95% of explants producing an average of 4.35 shoots and 3.29 nodes. The highest percentages of rooted explants (68%) and survival plants after acclimatization (81%) were achieved using shoots induced with 10.0&#xa0;mM IBA. This suggests that the culture medium and PGRs significantly influenced the morphogenic responses, leading to the development of an efficient micropropagation protocol for <i>C. paradoxa</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

In vitro propagation of Colletia paradoxa: an endangered species

  • 期刊论文

  • 发表日期：

    2026年1月30日

查看全文
  • Rhuann Carlo Viero Taques,
  • Rafaela Cristina Tome Souza,
  • Patricia Carla Giloni-Lima,
  • Vanderlei Aparecido de Lima,
  • Luciana Lopes Fortes Ribas

摘要

Colletia paradoxa is an endangered species with ornamental and medicinal potential. This study aimed to evaluate the effect of the culture medium and concentrations of plant growth regulators (PGRs) to establish an efficient protocol for plants production through in vitro cultivation. Nodal segments were cultured in MS, MS/2, MSM/2 and WPM media to determine the best culture medium. During the shoot development phase, the MS/2 medium was supplemented with 2.5, 5.0, and 10.0 µM of 6-benzylaminopurine (BA) and a control. The addition of 5.0 µM of cytokinins kinetin (KIN), zeatin (ZEA), and 2-isopentenyladenine (2IP) was tested. Rooting was assessed by soaking the base of the shoots in indole-3-butyric acid (IBA) solutions (2.5 mM, 5.0 mM, and 10.0 mM) for five minutes before transferring them to MS/2 medium. The best shoot development and nodal culture occurred with 5.0 µM BA, resulting in 95% of explants producing an average of 4.35 shoots and 3.29 nodes. The highest percentages of rooted explants (68%) and survival plants after acclimatization (81%) were achieved using shoots induced with 10.0 mM IBA. This suggests that the culture medium and PGRs significantly influenced the morphogenic responses, leading to the development of an efficient micropropagation protocol for C. paradoxa.