Scholarlink

<p>Dextran sulfate sodium (DSS) is widely used to induce experimental colitis in animal models, facilitating the investigation of inflammatory bowel disease (IBD). However, its strong polyanionic nature interferes with RNA extraction and suppresses reverse transcription and polymerase chain reaction (PCR) amplification, compromising downstream quantitative PCR (qPCR) analyses. Conventional RNA purification methods, such as lithium chloride precipitation and commercial kits, are limited by time, cost, and efficiency. This review outlines the key challenges associated with RNA isolation from DSS-treated tissues and presents an optimized extraction protocol integrating TRIzol with silica spin column purification. The method is systematically evaluated in mouse colonic tissues from DSS-induced colitis models, focusing on its compatibility with downstream qPCR analysis of pro-inflammatory cytokine expression.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Optimized TRIzol–silica column RNA extraction for DSS-colitis: resolving PCR inhibition

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年9月12日

查看全文
  • Jie Lin,
  • Jiacheng Zhang,
  • Adria Morales Santiago,
  • Quancai Sun

摘要

Dextran sulfate sodium (DSS) is widely used to induce experimental colitis in animal models, facilitating the investigation of inflammatory bowel disease (IBD). However, its strong polyanionic nature interferes with RNA extraction and suppresses reverse transcription and polymerase chain reaction (PCR) amplification, compromising downstream quantitative PCR (qPCR) analyses. Conventional RNA purification methods, such as lithium chloride precipitation and commercial kits, are limited by time, cost, and efficiency. This review outlines the key challenges associated with RNA isolation from DSS-treated tissues and presents an optimized extraction protocol integrating TRIzol with silica spin column purification. The method is systematically evaluated in mouse colonic tissues from DSS-induced colitis models, focusing on its compatibility with downstream qPCR analysis of pro-inflammatory cytokine expression.