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Key message <p><i>TaDJA-6D</i> boosts wheat regeneration and transformation efficiency across genotypes, offering a new molecular breeding resource.</p> Abstract <p>As a key member of the heat shock protein 40 family, the co-chaperone DnaJ proteins play a crucial role in plant growth, development, and stress responses. However, very few studies have focused on the function of DnaJ protein in wheat (<i>Triticum aestivum</i> L.). Here, we characterized a DnaJ family gene from wheat, <i>TaDJA-6D</i>, (<i>TraesCS6D02G232600</i>) and analyzed its spatiotemporal expression pattern. During wheat regeneration, the transcriptional expression level of the <i>TaDJA-6D</i> gene was continuously upregulated with the extension of tissue culture time; in situ hybridization revealed that the genes were highly expressed in the vigorously dividing regions of wheat calli. Functional analysis demonstrated that, compared with the control vector, overexpression of <i>TaDJA-6D</i> significantly improved the wheat regeneration and transformation efficiency across diverse wheat genotypes, including Fielder, Kenong 199, Yanda 1817 and Xiaoyan 6. Our findings suggest that <i>TaDJA-6D</i> plays an important role in the process of wheat regeneration and genetic transformation. This study deepens our understanding of the functions of wheat co-chaperone proteins and provides novel genetic resources for efficient wheat transformation.</p>

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TaDJA-6D, the DnaJ family gene, enhances the regeneration and transformation in wheat (Triticum aestivum L.)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月4日

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  • Menglu Li,
  • Yanyan Chang,
  • Xiaoting Shi,
  • Ying Song,
  • Mengyi Zhou,
  • Xian Sheng Zhang,
  • Xiao Min Bie

摘要

Key message

TaDJA-6D boosts wheat regeneration and transformation efficiency across genotypes, offering a new molecular breeding resource.

Abstract

As a key member of the heat shock protein 40 family, the co-chaperone DnaJ proteins play a crucial role in plant growth, development, and stress responses. However, very few studies have focused on the function of DnaJ protein in wheat (Triticum aestivum L.). Here, we characterized a DnaJ family gene from wheat, TaDJA-6D, (TraesCS6D02G232600) and analyzed its spatiotemporal expression pattern. During wheat regeneration, the transcriptional expression level of the TaDJA-6D gene was continuously upregulated with the extension of tissue culture time; in situ hybridization revealed that the genes were highly expressed in the vigorously dividing regions of wheat calli. Functional analysis demonstrated that, compared with the control vector, overexpression of TaDJA-6D significantly improved the wheat regeneration and transformation efficiency across diverse wheat genotypes, including Fielder, Kenong 199, Yanda 1817 and Xiaoyan 6. Our findings suggest that TaDJA-6D plays an important role in the process of wheat regeneration and genetic transformation. This study deepens our understanding of the functions of wheat co-chaperone proteins and provides novel genetic resources for efficient wheat transformation.