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<p>Hepatic ischemia–reperfusion (I/R) injury is a significant concern in liver transplantation and surgery. This study aimed to investigate the protective effects of pre-ischemic vitamin E (vit E; alpha-tocopherol) administration in a rat model using morphometric analysis. Although quantitative studies have demonstrated the protective effects of vit E, qualitative research utilizing stereological methods to assess its specific role in liver I/R injury remains scarce. Adult male Sprague-Dawley rats were divided into three groups: control, I/R, and vit E + I/R. The treatment group received 40&#xa0;mg/kg of vit E intraperitoneally once daily for seven days before ischemia. Following 45&#xa0;min of hepatic ischemia and 24&#xa0;h of reperfusion, liver tissues were analyzed histologically. The I/R group displayed significant liver damage, including irregular hepatocytes, dilated central veins, extensive foci of hepatocellular necrosis, and pyknotic hepatocyte nuclei. However, these abnormalities were less pronounced in the vit E + I/R group. Quantitative analysis indicated that the vit E + I/R group had significantly fewer pyknotic hepatocyte nuclei per unit area than the I/R group. Furthermore, the sinusoid-to-liver parenchyma volume ratio decreased from 36% in the I/R group to 32% in the vit E + I/R group, while the lobule area remained statistically unchanged. In conclusion, pre-ischemic administration of vit E attenuated histological and stereological indicators of hepatic I/R injury in this rat model. These findings support prophylactic alpha-tocopherol as a potential adjunctive strategy to mitigate hepatic I/R–related damage in the perioperative or pretransplant setting; however, further mechanistic and translational studies are warranted.</p> Graphical abstract <p></p>

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Stereological Assessment of the Protective Effect of Vitamin E (Alpha-Tocopherol) on Liver Ischemia–Reperfusion Injury in Rats

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月17日

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  • Paria Shojaolsadati,
  • Ferruh Yucel,
  • Semih Oz,
  • Dilek Burukoglu Donmez,
  • Hilmi Ozden

摘要

Hepatic ischemia–reperfusion (I/R) injury is a significant concern in liver transplantation and surgery. This study aimed to investigate the protective effects of pre-ischemic vitamin E (vit E; alpha-tocopherol) administration in a rat model using morphometric analysis. Although quantitative studies have demonstrated the protective effects of vit E, qualitative research utilizing stereological methods to assess its specific role in liver I/R injury remains scarce. Adult male Sprague-Dawley rats were divided into three groups: control, I/R, and vit E + I/R. The treatment group received 40 mg/kg of vit E intraperitoneally once daily for seven days before ischemia. Following 45 min of hepatic ischemia and 24 h of reperfusion, liver tissues were analyzed histologically. The I/R group displayed significant liver damage, including irregular hepatocytes, dilated central veins, extensive foci of hepatocellular necrosis, and pyknotic hepatocyte nuclei. However, these abnormalities were less pronounced in the vit E + I/R group. Quantitative analysis indicated that the vit E + I/R group had significantly fewer pyknotic hepatocyte nuclei per unit area than the I/R group. Furthermore, the sinusoid-to-liver parenchyma volume ratio decreased from 36% in the I/R group to 32% in the vit E + I/R group, while the lobule area remained statistically unchanged. In conclusion, pre-ischemic administration of vit E attenuated histological and stereological indicators of hepatic I/R injury in this rat model. These findings support prophylactic alpha-tocopherol as a potential adjunctive strategy to mitigate hepatic I/R–related damage in the perioperative or pretransplant setting; however, further mechanistic and translational studies are warranted.

Graphical abstract