Scholarlink

Background <p>Cutaneous melanoma, particularly the superficial spreading subtype, remains a diagnostic challenge because of its morphologic heterogeneity and subtle clinical manifestations. The immunohistochemical marker PRAME (Preferentially Expressed Antigen in Melanoma) has recently emerged as a potential adjunct in differentiating malignant from benign melanocytic lesions.</p> Aims <p>To evaluate PRAME expression patterns in superficial spreading melanoma and to explore correlations between PRAME-positive foci and specific dermoscopic features.</p> Methods <p>Twenty-two histologically confirmed superficial spreading melanomas exhibiting symmetrical dermoscopic patterns prior to excision were retrospectively analyzed. PRAME immunohistochemistry was performed on formalin-fixed, paraffin-embedded specimens using standardized protocols. Staining intensity and distribution were semiquantitatively scored (1–4). Five PRAME immunohistochemical hot spots per lesion were selected and retrospectively correlated with dermoscopic colors and structures.</p> Results <p>Diffuse PRAME expression was detected in 50% of cases and occurred significantly more often in invasive melanomas compared with melanoma in situ (<i>p</i> = 0.04). The predominant PRAME score was 3 (77.3%), followed by 4 (22.7%), with no lesions showing scores 1 or 2. PRAME expression did not correlate with the overall dermoscopic pattern or number of colors per lesion. However, high PRAME positivity within PRAME immunohistochemical hot spots most consistently corresponded to the blue-white veil, while regression structures, blotches, dots, chrysalis-like structures, and pseudonetwork (in one case) also showed strong associations.</p> Conclusion <p>Specific dermoscopic features may predict underlying PRAME overexpression, potentially improving pre-biopsy diagnostic stratification. These results support the adjunctive role of PRAME in integrated melanoma diagnostics, warranting confirmation in larger, standardized studies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

PRAME Immunohistochemical Positivity in Superficial Spreading Melanoma: Significance and Association with Melanoma Dermoscopy Features

  • 期刊论文

  • 发表日期：

    2026年3月3日

查看全文
  • Dunja Vesković,
  • Jelena Ilić Sabo,
  • Dejan Miljković,
  • Aleksandra M. Ilić,
  • Milana Vuković,
  • Tatjana Roš

摘要

Background

Cutaneous melanoma, particularly the superficial spreading subtype, remains a diagnostic challenge because of its morphologic heterogeneity and subtle clinical manifestations. The immunohistochemical marker PRAME (Preferentially Expressed Antigen in Melanoma) has recently emerged as a potential adjunct in differentiating malignant from benign melanocytic lesions.

Aims

To evaluate PRAME expression patterns in superficial spreading melanoma and to explore correlations between PRAME-positive foci and specific dermoscopic features.

Methods

Twenty-two histologically confirmed superficial spreading melanomas exhibiting symmetrical dermoscopic patterns prior to excision were retrospectively analyzed. PRAME immunohistochemistry was performed on formalin-fixed, paraffin-embedded specimens using standardized protocols. Staining intensity and distribution were semiquantitatively scored (1–4). Five PRAME immunohistochemical hot spots per lesion were selected and retrospectively correlated with dermoscopic colors and structures.

Results

Diffuse PRAME expression was detected in 50% of cases and occurred significantly more often in invasive melanomas compared with melanoma in situ (p = 0.04). The predominant PRAME score was 3 (77.3%), followed by 4 (22.7%), with no lesions showing scores 1 or 2. PRAME expression did not correlate with the overall dermoscopic pattern or number of colors per lesion. However, high PRAME positivity within PRAME immunohistochemical hot spots most consistently corresponded to the blue-white veil, while regression structures, blotches, dots, chrysalis-like structures, and pseudonetwork (in one case) also showed strong associations.

Conclusion

Specific dermoscopic features may predict underlying PRAME overexpression, potentially improving pre-biopsy diagnostic stratification. These results support the adjunctive role of PRAME in integrated melanoma diagnostics, warranting confirmation in larger, standardized studies.