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Objective <p>This study aimed to compare the in vitro effects of bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue mesenchymal stem cells (AT-MSCs) in mitigating intrauterine growth retardation caused by oxidative stress.</p> Materials and Method <p>9.5-day-old embryos from Wistar albino pregnant rats were exposed to in vitro hypoxia and treated with BM-MSCs or AT-MSCs in embryo culture. At 11.5&#xa0;days, embryos and yolk sacs were evaluated morphologically and histologically to assess developmental differences between groups.</p> Results <p>Hypoxia induced angiogenesis- and neurogenesis-related anomalies. Stem cell treatments (H + BM-MSC, H + AT-MSC) significantly improved embryonic development compared to the hypoxia group (<i>p</i> &lt; <i>0.05</i>). Although stem cell-treated embryos lagged slightly behind controls under normoxia (<i>p</i> &gt; <i>0.05</i>), both BM-MSC and AT-MSC applications mitigated hypoxia-related growth defects. Notably, the H + AT-MSC group showed superior development compared to the H + BM-MSC group (<i>p</i> &lt; <i>0.05</i>), with results closer to the normoxic control group.</p> Conclusion <p>AT-MSCs demonstrated a more effective improvement in embryonic and yolk sac development compared to BM-MSCs under hypoxic conditions. These findings suggest that AT-MSC therapy could offer a promising approach to treat angiogenetic and neurogenetic disorders caused by oxidative stress.</p>

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Effects of Stem Cells on Rat Embryo Development in Hypoxy Embryo Culture

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月7日

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  • Dilara Patat,
  • Mehtap Nisari,
  • Sümeyye Uçar,
  • Zeynep Burçin Gönen,
  • Şeyda Korkmaz,
  • Arzu Hanım Yay,
  • Özge Cengiz Mat,
  • Berin Tuğtağ Demir,
  • Özge Al,
  • Gökçen Dinç,
  • Seher Yılmaz

摘要

Objective

This study aimed to compare the in vitro effects of bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue mesenchymal stem cells (AT-MSCs) in mitigating intrauterine growth retardation caused by oxidative stress.

Materials and Method

9.5-day-old embryos from Wistar albino pregnant rats were exposed to in vitro hypoxia and treated with BM-MSCs or AT-MSCs in embryo culture. At 11.5 days, embryos and yolk sacs were evaluated morphologically and histologically to assess developmental differences between groups.

Results

Hypoxia induced angiogenesis- and neurogenesis-related anomalies. Stem cell treatments (H + BM-MSC, H + AT-MSC) significantly improved embryonic development compared to the hypoxia group (p < 0.05). Although stem cell-treated embryos lagged slightly behind controls under normoxia (p > 0.05), both BM-MSC and AT-MSC applications mitigated hypoxia-related growth defects. Notably, the H + AT-MSC group showed superior development compared to the H + BM-MSC group (p < 0.05), with results closer to the normoxic control group.

Conclusion

AT-MSCs demonstrated a more effective improvement in embryonic and yolk sac development compared to BM-MSCs under hypoxic conditions. These findings suggest that AT-MSC therapy could offer a promising approach to treat angiogenetic and neurogenetic disorders caused by oxidative stress.