Scholarlink

<p>Chilli (<i>Capsicum annuum</i> L.) cultivation is severely threatened by viral and phytoplasma pathogens, leading to significant yield losses. During a field survey at Tamil Nadu Agricultural University, Coimbatore, India, chilli plants showing typical begomovirus symptoms (upward leaf curling, crinkling) and phytoplasma-like symptoms (witches’ broom) were observed. Molecular assays confirmed the presence of Chilli leaf curl virus (<i>Begomovirus chillicapsici</i>- ChiLCV) and <i>‘Candidatus</i> Phytoplasma australasiaticum<i>’</i> in the tested samples. Sequence and phylogenetic analyses revealed close similarity of ChiLCV with isolates from Karnataka and Andhra Pradesh, while the phytoplasma clustered with the 16SrII-D subgroup. Virtual RFLP alaysis also further validated the subgroup classification. The findings of present study revealed the co-infection of <i>Ca</i>. P. australasiaticum and ChiLCV in chilli. The study underscores the emerging complexity of pathogen interactions and highlights the importance of molecular diagnostics in identifying mixed infections.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular characterization of Candidatus Phytoplasma australasiaticum strain causing mixed infection with chilli leaf curl virus (Begomovirus chillicapsici) on Capsicum annuum in India

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月16日

查看全文
  • J. Vinodhini,
  • L. Rajendran,
  • K. Nagendran,
  • G. Karthikeyan

摘要

Chilli (Capsicum annuum L.) cultivation is severely threatened by viral and phytoplasma pathogens, leading to significant yield losses. During a field survey at Tamil Nadu Agricultural University, Coimbatore, India, chilli plants showing typical begomovirus symptoms (upward leaf curling, crinkling) and phytoplasma-like symptoms (witches’ broom) were observed. Molecular assays confirmed the presence of Chilli leaf curl virus (Begomovirus chillicapsici- ChiLCV) and ‘Candidatus Phytoplasma australasiaticum’ in the tested samples. Sequence and phylogenetic analyses revealed close similarity of ChiLCV with isolates from Karnataka and Andhra Pradesh, while the phytoplasma clustered with the 16SrII-D subgroup. Virtual RFLP alaysis also further validated the subgroup classification. The findings of present study revealed the co-infection of Ca. P. australasiaticum and ChiLCV in chilli. The study underscores the emerging complexity of pathogen interactions and highlights the importance of molecular diagnostics in identifying mixed infections.