<p>Microbial biofilms are an essential component of plant-microbe interactions where rhizosphere is one such structure that provide nature’s creative answers for sustainable agriculture. The extracellular polymeric matrix surrounding biofilm communities promotes microbial colonization, nutrient cycling, and plant stress tolerance. Biofilms greatly contribute to plant development through various methods, such as altered root systems, enhanced nutrient availability, pathogen control, and abiotic stress reduction. Some important microbial genera that develop biofilm together or with others to benefit plant health and growth are <i>Trichoderma</i>,<i> Bacillus</i>, <i>Pseudomonas</i>, etc. Optimizing the agricultural application of microbial biofilm requires a detailed understanding of the molecular processes behind quorum sensing, biofilm development, and interactions between species. Advances in plant microbe biofilm research have been intensified in last decade that is clearly reflected in the microbe-based products. These developments have been further intensified by application of nanotechnology tools that have increased agricultural yield, lessen reliance on chemical inputs, and maintain environmental sustainability. This review has compiled the information about microbial biofilm as plant growth promoting means that is constructed either by single species or by mixed microbial community. The importance of quorum sensing and signaling molecules in formation of biofilm, its interaction with plant, has also been summarized. Further the commercial products developed for promoting microbial biofilm, resisting microbial attack, and fulfilling the nutrient requirements has also been reviewed. The review presents a comprehensive detail on biofilms that benefit plant as well as soil health and emphasizes on practicing/promoting application of microbial biofilms for developing robust and sustainable crop production systems.</p>