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<p>Medicinal plants have always been a non-exhaustible source of therapeutics throughout human history. Meanwhile, their in situ populations are under increasing anthropogenic pressure, specifically overharvesting and habitat destruction. Plant tissue culture is a powerful tool in biotechnological strategies of controlled selection, ex situ preservation and mass cloning of elite genotypes in medicine. The current study represents a study focusing on the development of an effective in vitro culture protocol of four important medicinal plant species: <i>Justicia adhatoda</i>,<i> Sida acuta</i>,<i> Pimenta dioica</i>, and <i>Premna integrifolia</i>. Induction of callus was achieved successfully by adding different concentrations of auxins and cytokinins to basal growth medium, especially 2,4-dichlorophenoxyacetic acid and Thidiazuron. Maximum callus formation was achieved in <i>J. adhatoda</i> (76.67%), <i>S. acuta</i> (88.33%), <i>P. dioica</i> (68.33%), and <i>P. integrifolia</i> (81.66%). Shoot regeneration was significantly enhanced by 6-benzylaminopurine, with responses of 62.22%, 73.33%, 65.55%, and 77.78% in the respective species. Root induction was effectively obtained with a combination of auxins, showing 72.22% in <i>P. integrifolia</i>, 79.99% in <i>S. acuta</i>, 84.44% in <i>J. adhatoda</i>, and 76.66% in <i>P. dioica</i>. The regenerated plantlets were acclimatized successfully under greenhouse conditions, confirming their survival and adaptability. The protocol showed very high efficiency in callus induction, shoot proliferation and root formation and therefore a valuable tool to be used in conservation, genetic improvement and sustained commercial exploitation of these medicinal species.</p>

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Development of an efficient in vitro system for micropropagation and regeneration of Justicia adhatoda, Sida acuta, Pimenta dioica, and Premna integrifolia

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月29日

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  • Marimuthu Poornalakshmi,
  • Jothi Kanmani Bharathi,
  • Siva Sankar Sai Prathyusha Neelam,
  • Panneerselvam Gurumoorthy,
  • Murugaiyan Divya,
  • Muthu Arjuna Samy Prakash,
  • Ramaswamy Anandan

摘要

Medicinal plants have always been a non-exhaustible source of therapeutics throughout human history. Meanwhile, their in situ populations are under increasing anthropogenic pressure, specifically overharvesting and habitat destruction. Plant tissue culture is a powerful tool in biotechnological strategies of controlled selection, ex situ preservation and mass cloning of elite genotypes in medicine. The current study represents a study focusing on the development of an effective in vitro culture protocol of four important medicinal plant species: Justicia adhatoda, Sida acuta, Pimenta dioica, and Premna integrifolia. Induction of callus was achieved successfully by adding different concentrations of auxins and cytokinins to basal growth medium, especially 2,4-dichlorophenoxyacetic acid and Thidiazuron. Maximum callus formation was achieved in J. adhatoda (76.67%), S. acuta (88.33%), P. dioica (68.33%), and P. integrifolia (81.66%). Shoot regeneration was significantly enhanced by 6-benzylaminopurine, with responses of 62.22%, 73.33%, 65.55%, and 77.78% in the respective species. Root induction was effectively obtained with a combination of auxins, showing 72.22% in P. integrifolia, 79.99% in S. acuta, 84.44% in J. adhatoda, and 76.66% in P. dioica. The regenerated plantlets were acclimatized successfully under greenhouse conditions, confirming their survival and adaptability. The protocol showed very high efficiency in callus induction, shoot proliferation and root formation and therefore a valuable tool to be used in conservation, genetic improvement and sustained commercial exploitation of these medicinal species.