Scholarlink

<p><i>Nicotiana tabacum</i> (PT76 variety) is a major source of bioactive compounds, yet its chemical composition undergoes substantial transformation during post-harvest curing. This study aimed to compare the phytochemical profiles of green (GL) and cured tobacco leaves (CL) consumed locally as <i>khaini</i> in Bihar and Jharkhand, using both qualitative phytochemical screening and Gas Chromatography–Mass Spectrometry (GC–MS). Aqueous extracts of green and cured leaves were subjected to standard phytochemical tests, revealing the presence of alkaloids, flavonoids, glycosides, phenolic compounds, proteins, carbohydrates, anthocyanidins, phlobatannins, terpenoids, saponins, and steroids. Methanolic extracts of both samples were analyzed, and compounds were identified based on retention times and spectral matching with the NIST and Wiley library. The cured leaf extract exhibited 42 compounds, with (S)-3-(1-methylpyrrolidin-2-yl)pyridine (nicotine 88.83%) as the dominant constituent, along with potentially harmful compounds such as 1,2,3,6-tetrahydro-2,3′-bipyridine (1.19%), 4-nitroso-3-(pyrrolidin-1-yl)phenol (0.38%), and 1-methyl-5-(pyridin-3-yl)pyrrolidin-2-one (0.80%). In contrast, the green leaf extract contained 25 compounds including nicotine, naphthalene, 1,2,3,6-tetrahydro-2,3′-bipyridine, neophytadiene, phytol, stigmasterol, β-sitosterol, hexadecanoic acid methyl ester, methyl stearate, and 9,12-octadecadienoic acid methyl ester. Additional compounds include sclareolide, palmitic acid derivatives, and various long-chain hydrocarbons and esters. These findings indicate that curing significantly alters the chemical landscape of <i>N. tabacum</i> leaves, increasing the presence of toxicants and nitrosamine precursors. The study provides chemical evidence supporting the toxicological concerns associated with cured tobacco products and highlights the need for public health awareness regarding the risks of smokeless tobacco consumption.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Comparative GC-MS and phytochemical profiling of green and cured leaves of Nicotiana tabacum (PT76) consumed as Khaini in Jharkhand

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月3日

查看全文
  • Swati Sandhya,
  • Shalini Mehta,
  • Vinay Oraon

摘要

Nicotiana tabacum (PT76 variety) is a major source of bioactive compounds, yet its chemical composition undergoes substantial transformation during post-harvest curing. This study aimed to compare the phytochemical profiles of green (GL) and cured tobacco leaves (CL) consumed locally as khaini in Bihar and Jharkhand, using both qualitative phytochemical screening and Gas Chromatography–Mass Spectrometry (GC–MS). Aqueous extracts of green and cured leaves were subjected to standard phytochemical tests, revealing the presence of alkaloids, flavonoids, glycosides, phenolic compounds, proteins, carbohydrates, anthocyanidins, phlobatannins, terpenoids, saponins, and steroids. Methanolic extracts of both samples were analyzed, and compounds were identified based on retention times and spectral matching with the NIST and Wiley library. The cured leaf extract exhibited 42 compounds, with (S)-3-(1-methylpyrrolidin-2-yl)pyridine (nicotine 88.83%) as the dominant constituent, along with potentially harmful compounds such as 1,2,3,6-tetrahydro-2,3′-bipyridine (1.19%), 4-nitroso-3-(pyrrolidin-1-yl)phenol (0.38%), and 1-methyl-5-(pyridin-3-yl)pyrrolidin-2-one (0.80%). In contrast, the green leaf extract contained 25 compounds including nicotine, naphthalene, 1,2,3,6-tetrahydro-2,3′-bipyridine, neophytadiene, phytol, stigmasterol, β-sitosterol, hexadecanoic acid methyl ester, methyl stearate, and 9,12-octadecadienoic acid methyl ester. Additional compounds include sclareolide, palmitic acid derivatives, and various long-chain hydrocarbons and esters. These findings indicate that curing significantly alters the chemical landscape of N. tabacum leaves, increasing the presence of toxicants and nitrosamine precursors. The study provides chemical evidence supporting the toxicological concerns associated with cured tobacco products and highlights the need for public health awareness regarding the risks of smokeless tobacco consumption.