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<p><i>Schisandra sphenanthera</i> Rehder &amp; E. H. Wilson 1913, endemic to the temperate areas of China, is a perennial deciduous woody vine used in traditional Chinese medicine and horticulture. Here, the complete chloroplast sequence of <i>S. sphenanthera</i> was sequenced and assembled. The new chloroplast genome is 146,843&#xa0;bp in length, including a small single-copy region (SSC) of 18,281&#xa0;bp, a large single-copy region (LSC) of 95,628&#xa0;bp, and a pair of inverted repeat (IR) regions, each with a length of 16,314&#xa0;bp. A total of 113 predicted genes containing 79 protein-coding genes, four ribosomal RNA genes and 30 transfer RNA genes were annotated. We also identified 47 microsatellite repeat sequences and 100 tandem repeat sequences in the chloroplast genome of <i>S. sphenanthera</i>. According to the result of the phylogenetic analysis, <i>S. sphenanthera</i> and <i>S. chinensis</i> are closely related, and both species belong to the Austrobaileyales. (the third clade of early-diverging angiosperm), immediately after <i>Amborella</i> and <i>Nuphar</i>. Four single base differences and three copy number changes in SSR between two <i>S. sphenanthera</i> individual chloroplast genomes indicated that the copy number of SSRs would be one of mechanisms of intraspecies chloroplast differentiation. This study would provide a reliable genetic and evolutionary resource, offering a deeper insight into the intraspecies diversity within chloroplast genome.</p>

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Characterization and intraspecies variation of the complete chloroplast genome of Schisandra sphenanthera, an important medical plant

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  • 发表日期：

    2025年3月21日

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  • Xianxian Yu,
  • Wei Zhai,
  • Yajun Wang,
  • Guoxi Wu,
  • Jie Zhang

摘要

Schisandra sphenanthera Rehder & E. H. Wilson 1913, endemic to the temperate areas of China, is a perennial deciduous woody vine used in traditional Chinese medicine and horticulture. Here, the complete chloroplast sequence of S. sphenanthera was sequenced and assembled. The new chloroplast genome is 146,843 bp in length, including a small single-copy region (SSC) of 18,281 bp, a large single-copy region (LSC) of 95,628 bp, and a pair of inverted repeat (IR) regions, each with a length of 16,314 bp. A total of 113 predicted genes containing 79 protein-coding genes, four ribosomal RNA genes and 30 transfer RNA genes were annotated. We also identified 47 microsatellite repeat sequences and 100 tandem repeat sequences in the chloroplast genome of S. sphenanthera. According to the result of the phylogenetic analysis, S. sphenanthera and S. chinensis are closely related, and both species belong to the Austrobaileyales. (the third clade of early-diverging angiosperm), immediately after Amborella and Nuphar. Four single base differences and three copy number changes in SSR between two S. sphenanthera individual chloroplast genomes indicated that the copy number of SSRs would be one of mechanisms of intraspecies chloroplast differentiation. This study would provide a reliable genetic and evolutionary resource, offering a deeper insight into the intraspecies diversity within chloroplast genome.