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<p><i>Taraxacum kok-saghyz</i> dandelion is a leading alternative natural rubber-producing plant that thrives in temperate climates. Latex, rubber and rubber transferase (RTase) activity of genetically identical clonal plants, produced in tissue culture, and then grown in the field for 6&#xa0;months to 1&#xa0;year were quantified throughout development. Induction of rubber transferase activity and rubber accumulation occurred between three and 6&#xa0;weeks old. RTase activity increased with age up to 16&#xa0;weeks, while latex concentration increased up to week 20. When enzymatically active WRP were saturated with 5&#xa0;mM [<sup>14</sup>C]-isopentenyl pyrophosphate (IPP) and 12&#xa0;µM farnesyl pyrophosphate (FPP), reaction velocity was 85% higher at 16&#xa0;weeks than at 12&#xa0;weeks of plant growth. The RTase FPP K<sub>m</sub> was about 0.263&#xa0;µM, through most of the growth period, in line with other laticiferous species. High molecular weight rubber was produced in very young roots. 0.9&#xa0;Mg/mol in only 6&#xa0;weeks and 1.1&#xa0;Mg/mol in 8&#xa0;weeks. A rubber molecular weight exceeding 1&#xa0;Mg/mol indicates that dandelion is a viable alternative rubber-producing plant that can be cultivated in the U.S. to support the domestic rubber industry.</p> Graphical Abstract <p></p>

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Rubber accumulation and rubber transferase activity during root development of Taraxacum kok-saghyz dandelion

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月6日

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  • Beenish Saba,
  • Deborah J. Scott,
  • Colleen McMahan,
  • David Shintani,
  • Katrina Cornish

摘要

Taraxacum kok-saghyz dandelion is a leading alternative natural rubber-producing plant that thrives in temperate climates. Latex, rubber and rubber transferase (RTase) activity of genetically identical clonal plants, produced in tissue culture, and then grown in the field for 6 months to 1 year were quantified throughout development. Induction of rubber transferase activity and rubber accumulation occurred between three and 6 weeks old. RTase activity increased with age up to 16 weeks, while latex concentration increased up to week 20. When enzymatically active WRP were saturated with 5 mM [14C]-isopentenyl pyrophosphate (IPP) and 12 µM farnesyl pyrophosphate (FPP), reaction velocity was 85% higher at 16 weeks than at 12 weeks of plant growth. The RTase FPP Km was about 0.263 µM, through most of the growth period, in line with other laticiferous species. High molecular weight rubber was produced in very young roots. 0.9 Mg/mol in only 6 weeks and 1.1 Mg/mol in 8 weeks. A rubber molecular weight exceeding 1 Mg/mol indicates that dandelion is a viable alternative rubber-producing plant that can be cultivated in the U.S. to support the domestic rubber industry.

Graphical Abstract