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<p>This study investigates the chemistry of bioplastic production by <i>Alcaligenes faecalis</i> strain NSBN10 using cane molasses as a low-cost carbon source. The bacterium capable of synthesizing polyhydroxybutyrate (PHB), a bioplastic, was screened using Sudan Black B dye and Nile Blue A staining, with the highest fluorescence level being observed for <i>A. faecalis</i> NSBN10. This strain was further identified through 16S rRNA gene sequencing. To optimize the bioplastic production, a statistically-based response surface methodology (RSM) was employed using a central composite design. The effects of cane molasses concentration (10–40&#xa0;g/L), yeast extract content (0.1–2&#xa0;g/L), and pH (6–9) on bioplastic yield and productivity were evaluated. The optimal conditions for the highest bioplastic yield (78.70%) were achieved with cane molasses at 25&#xa0;g/L, yeast extract at 1.05&#xa0;g/L, and a pH of 7.5, resulting in enhanced productivity. Fourier transform infrared spectroscopy (FTIR) analysis was used to characterize the bioplastic, confirming its composition. This study highlights the potential of utilizing cane molasses for cost-effective bioplastic production, with a focus on the underlying chemistry of the synthesis process.</p>

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Chemistry of bioplastic production by Alcaligenes faecalis NSBN10 using cane molasses: optimization and characterization

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月13日

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  • Nidhi Sharma,
  • Baljeet Singh Saharan

摘要

This study investigates the chemistry of bioplastic production by Alcaligenes faecalis strain NSBN10 using cane molasses as a low-cost carbon source. The bacterium capable of synthesizing polyhydroxybutyrate (PHB), a bioplastic, was screened using Sudan Black B dye and Nile Blue A staining, with the highest fluorescence level being observed for A. faecalis NSBN10. This strain was further identified through 16S rRNA gene sequencing. To optimize the bioplastic production, a statistically-based response surface methodology (RSM) was employed using a central composite design. The effects of cane molasses concentration (10–40 g/L), yeast extract content (0.1–2 g/L), and pH (6–9) on bioplastic yield and productivity were evaluated. The optimal conditions for the highest bioplastic yield (78.70%) were achieved with cane molasses at 25 g/L, yeast extract at 1.05 g/L, and a pH of 7.5, resulting in enhanced productivity. Fourier transform infrared spectroscopy (FTIR) analysis was used to characterize the bioplastic, confirming its composition. This study highlights the potential of utilizing cane molasses for cost-effective bioplastic production, with a focus on the underlying chemistry of the synthesis process.