<p>The rapid advancement of AI technology has significantly impacted higher education, presenting both opportunities and challenges for teaching and learning. Although students can benefit from AI, many remain unaware of its potential utility. Moreover, concerns regarding the accuracy and reliability of AI complicate its proper use. This study incorporated an AI teaching assistant, called Blueink, into a biochemistry course at a medical university in China. The researchers assessed the alterations in individuals’ knowledge, AI use, and critical thinking skills by conducting a review before and after the training. This was done to furnish valuable information for academics and specialists. The findings presented that the participating college students perceived AI as increasingly essential for contemporary learning and excelled at discovering significant facts using AI techniques. However, their confidence in AI responses and their habits and preferences for posing inquiries remained unchanged after the training. The study indicates that AI tools not only enhance students’ skill acquisition but require greater clarity and proficiency. Collaborating with diverse specialists can yield superior AI tools for education.</p>