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<p>The oral microbiome is central to the maintenance of oral health, and disruptions in its composition contribute to persistent infections, including endodontic pathologies. Among bacteria and other members of the microbial community, <i>Olsenella uli</i> is a concerning pathogen responsible for several endodontic infections. Despite its clinical significance, organism-specific therapeutic targets for <i>O. uli</i> remain largely unexplored. In this study, a systematic comparative subtractive proteomics pipeline was employed to identify novel drug targets unique to <i>O. uli</i> DSM 7084. Comparative KEGG pathway mapping revealed 21 pathogen-specific metabolic pathways, from which 14 non-homologous essential proteins were identified. Physicochemical characterization further priortized these to eight stable proteins prioritized for druggability screening. Druggability analysis using DrugBank identified corresponding FDA-approved compounds, and three-dimensional structures generated through I-TASSER enabled downstream molecular docking. Docking simulations highlighted three high-affinity protein–drug complexes—UDP-N-acetylmuramoylalanine–D-glutamate ligase, alanine racemase, and UDP-N-acetylglucosamine-1-carboxyvinyltransferase—with strong inhibitory potential. These findings provide a comprehensive in silico foundation for the rapid development of targeted antimicrobial strategies against <i>O. uli</i>, supporting future experimental validation for improved management of persistent oral and endodontic infections.</p>

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Exploration of the proteome of Olsenella uli DSM 7084 to identify potential drug targets against oral infections

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月8日

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  • Mohammad Salman Akhtar,
  • Arshi Talat,
  • Tulika Bhardwaj,
  • Mansoor Alsahag,
  • Ali Alisaac,
  • Nada M. Ali,
  • Abrar Ahmad,
  • Mazin A. Zamzami,
  • Hani Choudhry,
  • Ishtiaq Qadri

摘要

The oral microbiome is central to the maintenance of oral health, and disruptions in its composition contribute to persistent infections, including endodontic pathologies. Among bacteria and other members of the microbial community, Olsenella uli is a concerning pathogen responsible for several endodontic infections. Despite its clinical significance, organism-specific therapeutic targets for O. uli remain largely unexplored. In this study, a systematic comparative subtractive proteomics pipeline was employed to identify novel drug targets unique to O. uli DSM 7084. Comparative KEGG pathway mapping revealed 21 pathogen-specific metabolic pathways, from which 14 non-homologous essential proteins were identified. Physicochemical characterization further priortized these to eight stable proteins prioritized for druggability screening. Druggability analysis using DrugBank identified corresponding FDA-approved compounds, and three-dimensional structures generated through I-TASSER enabled downstream molecular docking. Docking simulations highlighted three high-affinity protein–drug complexes—UDP-N-acetylmuramoylalanine–D-glutamate ligase, alanine racemase, and UDP-N-acetylglucosamine-1-carboxyvinyltransferase—with strong inhibitory potential. These findings provide a comprehensive in silico foundation for the rapid development of targeted antimicrobial strategies against O. uli, supporting future experimental validation for improved management of persistent oral and endodontic infections.