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<p>Due to a lack of resources, the diagnosis of Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a challenge in Burkina Faso and West Africa, making MRSA surveillance difficult. In our study, we included 33 beta-lactam resistant <i>S. aureus</i> isolates from the Ouagadougou region which we further tested for susceptibility to cefoxitin using standard methods. Isolates resistant to cefoxitin were further investigated using Chrom MRSA ID<sup>®</sup> agar medium. In addition, genetic determinants including the <i>mecA</i> gene and <i>SCCmec</i> cassettes were searched using conventional PCR. Fourteen isolates (42.42%) were resistant to cefoxitin, out of which six (18.18%) tested positive when using the Chrom MRSA ID<sup>®</sup> agar medium. Six <i>S. aureus</i> isolates (18.18%) tested positive for <i>mecA</i>, and seven (21.21%) tested positive for the <i>SCCmec</i> IVa cassette. This study described circulating MRSA in the city of Ouagadougou. These MRSA harbor <i>mecA</i> resistance genes and <i>SCCmec</i> IVa cassettes.</p>

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Screening of mecA and SCCmec genes in Staphylococcus aureus from human and hospital wastewater origins in Ouagadougou, Burkina Faso

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月12日

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  • Ganamé Abasse Ouédraogo,
  • Kaboré Boukaré,
  • Roukiatou Traoré,
  • Henri Sidabéwindin Ouédraogo,
  • Hama Cissé,
  • Ismael Henri Nestor Bassolé,
  • François Tchoumbougnang,
  • Savadogo Aly

摘要

Due to a lack of resources, the diagnosis of Methicillin-resistant Staphylococcus aureus (MRSA) is a challenge in Burkina Faso and West Africa, making MRSA surveillance difficult. In our study, we included 33 beta-lactam resistant S. aureus isolates from the Ouagadougou region which we further tested for susceptibility to cefoxitin using standard methods. Isolates resistant to cefoxitin were further investigated using Chrom MRSA ID® agar medium. In addition, genetic determinants including the mecA gene and SCCmec cassettes were searched using conventional PCR. Fourteen isolates (42.42%) were resistant to cefoxitin, out of which six (18.18%) tested positive when using the Chrom MRSA ID® agar medium. Six S. aureus isolates (18.18%) tested positive for mecA, and seven (21.21%) tested positive for the SCCmec IVa cassette. This study described circulating MRSA in the city of Ouagadougou. These MRSA harbor mecA resistance genes and SCCmec IVa cassettes.