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<p>The interaction between single-molecule (SM) fluorescence and transparent conductive oxide interface presents unique opportunities for studying molecular motion dynamics and conformational changes. In this study, we investigate the quenching effect of indium-tin oxide (ITO) on SM fluorescence, focusing on the fluorescent dye Cy3 tethered to the 3′-end of single-stranded DNA (ssDNA). By examining the brightness variations of single Cy3 molecules, we are able to distinguish Cy3-ssDNA covalently attached onto the ITO surface from the case of adsorption. Additionally, we can evaluate the molecular motion dynamics of single ssDNA molecules of varying lengths and conformations on the ITO surface. We believe that our findings make significant contributions to the understanding of molecular interactions at ITO interfaces and offer valuable insights into the potential applications of novel fluorophore motion- and orientation-based biosensing strategies.</p>

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Investigating single-molecule fluorescence quenching and molecular motion dynamics at transparent conductive oxide interfaces

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月12日

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  • Zihan Pan,
  • Jin Lu

摘要

The interaction between single-molecule (SM) fluorescence and transparent conductive oxide interface presents unique opportunities for studying molecular motion dynamics and conformational changes. In this study, we investigate the quenching effect of indium-tin oxide (ITO) on SM fluorescence, focusing on the fluorescent dye Cy3 tethered to the 3′-end of single-stranded DNA (ssDNA). By examining the brightness variations of single Cy3 molecules, we are able to distinguish Cy3-ssDNA covalently attached onto the ITO surface from the case of adsorption. Additionally, we can evaluate the molecular motion dynamics of single ssDNA molecules of varying lengths and conformations on the ITO surface. We believe that our findings make significant contributions to the understanding of molecular interactions at ITO interfaces and offer valuable insights into the potential applications of novel fluorophore motion- and orientation-based biosensing strategies.