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<p>Thermochemolysis-gas chromatography–mass spectrometry (Thermochemolysis-GC–MS) was applied to detect fatty acid oxidation products in a single <i>Drosophila melanogaster</i> individual (dry weight: 100–400&#xa0;µg). Linoleic acid oxidized under UV irradiation was first analyzed to identify characteristic oxidation products, including azelaic acid. Using tetramethylammonium hydroxide (TMAH) as an organic alkali reagent, azelaic acid was successfully detected as its dimethyl ester in <i>D. melanogaster</i> individuals reared on linoleic acid–supplemented diets. The use of <i>m/z</i> 185 as a diagnostic ion in extracted ion chromatograms allowed for sensitive determination of azelaic acid in control and linoleic acid–treated <i>D. melanogaster</i> individuals. The treated groups exhibited notable increases in azelaic acid content, which demonstrates the method’s ability to evaluate statistically significant differences based on individual-level data.</p> Graphical abstract <p></p>

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Thermochemolysis-gas chromatographic detection of oxidized fatty acids in a single Drosophila melanogaster individual

  • 期刊论文

  • 发表日期：

    2025年12月16日

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  • Yasuyuki Ishida,
  • Misaki Haba,
  • Noa Komoda,
  • Nobuo Kido,
  • Atsushi Watanabe

摘要

Thermochemolysis-gas chromatography–mass spectrometry (Thermochemolysis-GC–MS) was applied to detect fatty acid oxidation products in a single Drosophila melanogaster individual (dry weight: 100–400 µg). Linoleic acid oxidized under UV irradiation was first analyzed to identify characteristic oxidation products, including azelaic acid. Using tetramethylammonium hydroxide (TMAH) as an organic alkali reagent, azelaic acid was successfully detected as its dimethyl ester in D. melanogaster individuals reared on linoleic acid–supplemented diets. The use of m/z 185 as a diagnostic ion in extracted ion chromatograms allowed for sensitive determination of azelaic acid in control and linoleic acid–treated D. melanogaster individuals. The treated groups exhibited notable increases in azelaic acid content, which demonstrates the method’s ability to evaluate statistically significant differences based on individual-level data.

Graphical abstract