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<p>The potential risks posed by antibiotics to human health and the ecological environment have long been a major public concern. Consequently, developing probe technologies capable of efficiently detecting trace levels of antibiotics in environments has emerged as a critical research priority. In this study, a conjugated fluorene-based pyridine diimine polymeric probe (FPD) was employed as a fluorescent probe for the specific recognition and detection of furaltadone. Experimental results showed that FPD rapidly interacts with furaltadone, resulting in a significant fluorescence quenching of FPD, and its fluorescence intensity could quickly reach a stable state. In the ethanol-PBS mixed solution system, the fluorescence quenching efficiency of FPD exhibits an excellent linear relationship with furaltadone concentrations ranging from 0 to 12&#xa0;µM, achieving a detection limit as low as 0.0490&#xa0;µM. In the presence of interfering substances, FPD maintains superior selectivity and anti-interference capability. Furthermore, a recovery experiment was conducted by spiking furaltadone into egg white, yielding recovery rates ranging from 97.29% to 104.22%, with a relative standard deviation (RSD) of less than 3.73%. This study develops a novel and reliable method for the efficient and precise detection of furaltadone, demonstrating broad application potential.</p> Graphical abstract <p></p>

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A conjugated pyridine diimine fluorene-based polymer for the detection of furaltadone

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • Lindan Gong,
  • Yan Sun,
  • Hongju Zhang,
  • Junqing Li,
  • Aohui Qin,
  • Lu-an Fan

摘要

The potential risks posed by antibiotics to human health and the ecological environment have long been a major public concern. Consequently, developing probe technologies capable of efficiently detecting trace levels of antibiotics in environments has emerged as a critical research priority. In this study, a conjugated fluorene-based pyridine diimine polymeric probe (FPD) was employed as a fluorescent probe for the specific recognition and detection of furaltadone. Experimental results showed that FPD rapidly interacts with furaltadone, resulting in a significant fluorescence quenching of FPD, and its fluorescence intensity could quickly reach a stable state. In the ethanol-PBS mixed solution system, the fluorescence quenching efficiency of FPD exhibits an excellent linear relationship with furaltadone concentrations ranging from 0 to 12 µM, achieving a detection limit as low as 0.0490 µM. In the presence of interfering substances, FPD maintains superior selectivity and anti-interference capability. Furthermore, a recovery experiment was conducted by spiking furaltadone into egg white, yielding recovery rates ranging from 97.29% to 104.22%, with a relative standard deviation (RSD) of less than 3.73%. This study develops a novel and reliable method for the efficient and precise detection of furaltadone, demonstrating broad application potential.

Graphical abstract