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<p>In this study, we proposed a novel detection concept using aptamer DNA based on particle dissociation in a coupled acoustic-gravitational (CAG) field. When the CAG field was formed in the channel, the PS particles on the glass plate exerted the acoustic radiation force (<i>F</i><sub>ac</sub>), sedimentation force (<i>F</i><sub>sed</sub>), and binding force between the PS particle and glass plate (<i>F</i><sub>bind</sub>). The applied voltage to the transducer (<i>V</i>) required for particle dissociation from the glass plate depends on the <i>F</i><sub>bind</sub>. When the arginine is introduced to the system, in which the PS particles are immobilized onto the glass plate through the DNA hybridization using aptamer for arginine, the change in <i>F</i><sub>bind</sub> can be induced, allowing us to detect the arginine concentration as the change in <i>V</i>. Thus, we demonstrated a detection concept for arginine that enables rough concentration estimation despite the limited precision.</p> Graphical abstract <p></p>

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Detection scheme of arginine as aptamer target based on particle dissociation in coupled acoustic-gravitational field

  • 期刊论文

  • 发表日期：

    2025年6月4日

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  • Akihisa Miyagawa,
  • Koki Nakano,
  • Kengo Oshiyama,
  • Shigenori Nagatomo,
  • Kiyoharu Nakatani,
  • Shoji Ishizaka

摘要

In this study, we proposed a novel detection concept using aptamer DNA based on particle dissociation in a coupled acoustic-gravitational (CAG) field. When the CAG field was formed in the channel, the PS particles on the glass plate exerted the acoustic radiation force (Fac), sedimentation force (Fsed), and binding force between the PS particle and glass plate (Fbind). The applied voltage to the transducer (V) required for particle dissociation from the glass plate depends on the Fbind. When the arginine is introduced to the system, in which the PS particles are immobilized onto the glass plate through the DNA hybridization using aptamer for arginine, the change in Fbind can be induced, allowing us to detect the arginine concentration as the change in V. Thus, we demonstrated a detection concept for arginine that enables rough concentration estimation despite the limited precision.

Graphical abstract