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<p>A ratiometric electrochemical aptasensor was constructed based on rGO-cMWCNTs/AuNPs functionalized graphene nanocomposites for the detection of CA19-9 in serum samples. First, rGO-cMWCNTs/AuNPs nanocomposites were modified on the surface of glassy carbon electrodes. Second, the aptamer complementary chain labeled with anthraquinone-2-carboxylic acid (cDNA-AQ) was covalently linked by Au–S. Finally, the hybrid chain was formed with ferrocene (Fc)-labeled Apt-Fc by base pairing, and a sensing interface with specific target recognition and electrochemical signal amplification was constructed. In this study, the ratio of response signal to internal reference signal (<i>I</i><sub>Fc</sub>/<i>I</i><sub>AQ</sub>) was used as the target molecular response signal to achieve the quantitative detection of CA19-9. The ratiometric electrochemical biosensor based on aptamer molecular recognition has good selectivity, reproducibility and stability. Under the optimal reaction conditions, the logarithm of CA19-9 concentration had a good linear relationship with <i>I</i><sub>Fc</sub>/<i>I</i><sub>AQ</sub>, the linear range was 1.0–1.0 × 10<sup>6</sup>&#xa0;mU/mL, the detection limit was 0.65&#xa0;mU/mL, and the recovery rate in CA19-9 serum was 94.12–97.83%. The results show that the sensor has potential practical application prospects, and provides a simple and feasible strategy for the rapid and accurate detection of other tumor markers.</p> Graphical abstract

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Ratiometric electrochemical aptasensor based on functionalized graphene nanocomposites for detection of CA19-9

  • 期刊论文

  • 发表日期：

    2025年3月6日

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  • Feifei Lin,
  • Xiaosha Li,
  • Jian Zhang,
  • Hailin Zhang,
  • Zemiao Zhang,
  • Lijie Hou,
  • Bingjun Shen,
  • Lihong Jin

摘要

A ratiometric electrochemical aptasensor was constructed based on rGO-cMWCNTs/AuNPs functionalized graphene nanocomposites for the detection of CA19-9 in serum samples. First, rGO-cMWCNTs/AuNPs nanocomposites were modified on the surface of glassy carbon electrodes. Second, the aptamer complementary chain labeled with anthraquinone-2-carboxylic acid (cDNA-AQ) was covalently linked by Au–S. Finally, the hybrid chain was formed with ferrocene (Fc)-labeled Apt-Fc by base pairing, and a sensing interface with specific target recognition and electrochemical signal amplification was constructed. In this study, the ratio of response signal to internal reference signal (IFc/IAQ) was used as the target molecular response signal to achieve the quantitative detection of CA19-9. The ratiometric electrochemical biosensor based on aptamer molecular recognition has good selectivity, reproducibility and stability. Under the optimal reaction conditions, the logarithm of CA19-9 concentration had a good linear relationship with IFc/IAQ, the linear range was 1.0–1.0 × 106 mU/mL, the detection limit was 0.65 mU/mL, and the recovery rate in CA19-9 serum was 94.12–97.83%. The results show that the sensor has potential practical application prospects, and provides a simple and feasible strategy for the rapid and accurate detection of other tumor markers.

Graphical abstract