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Background and aim <p>Carotid artery stenosis (CAS) is a significant contributor to cerebral infarction. miR-15b-3p shows aberrant expression in various diseases. This research sought to assess serum miR-15b-3p expression in CAS, its diagnostic value, and the underlying mechanisms.</p> Methods <p>This study recruited 111 CAS patients and 157 healthy controls. miR-15b-3p’s diagnostic potential was assessed using receiver operating characteristic (ROC) curve. The role of miR-15b-3p for human vascular smooth muscle cells (HA-VSMCs) was examined using cell transfection, proliferation assays, and apoptosis analysis. Targets of miR-15b-3p were identified through bioinformatics analysis and validated using dual-luciferase assays.</p> Results <p>Patients with CAS exhibited significantly elevated serum miR-15b-3p levels relative to controls. miR-15b-3p demonstrated 75.68% sensitivity and 81.82% specificity in CAS detection (AUC = 0.853) and could distinguish severe CAS (AUC = 0.788, sensitivity = 74.51%, specificity = 76.67%). miR-15b-3p promoted proliferation and inhibited apoptosis of HA-VSMCs by targeting PTEN. In CAS patients, PTEN showed a negative correlation with miR-15b-3p levels (<i>r</i> = -0.601, <i>P</i> &lt; 0.001).</p> Conclusion <p>Serum miR-15b-3p is elevated in CAS patients. It promotes the proliferation of HA-VSMC cells via targeting PTEN. Although miR-15b-3p holds promise diagnostic value for CAS, it is not yet sufficient for clinical diagnosis.</p>

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Serum miR-15b-3p as a Potential Diagnostic Biomarker for Carotid Artery Stenosis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月1日

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  • Yikun Jiang,
  • Nan Zhang,
  • Tong Huang,
  • Cheng Liao,
  • Tao Di,
  • Ahmet Matturdi,
  • Yingxue Hao

摘要

Background and aim

Carotid artery stenosis (CAS) is a significant contributor to cerebral infarction. miR-15b-3p shows aberrant expression in various diseases. This research sought to assess serum miR-15b-3p expression in CAS, its diagnostic value, and the underlying mechanisms.

Methods

This study recruited 111 CAS patients and 157 healthy controls. miR-15b-3p’s diagnostic potential was assessed using receiver operating characteristic (ROC) curve. The role of miR-15b-3p for human vascular smooth muscle cells (HA-VSMCs) was examined using cell transfection, proliferation assays, and apoptosis analysis. Targets of miR-15b-3p were identified through bioinformatics analysis and validated using dual-luciferase assays.

Results

Patients with CAS exhibited significantly elevated serum miR-15b-3p levels relative to controls. miR-15b-3p demonstrated 75.68% sensitivity and 81.82% specificity in CAS detection (AUC = 0.853) and could distinguish severe CAS (AUC = 0.788, sensitivity = 74.51%, specificity = 76.67%). miR-15b-3p promoted proliferation and inhibited apoptosis of HA-VSMCs by targeting PTEN. In CAS patients, PTEN showed a negative correlation with miR-15b-3p levels (r = -0.601, P < 0.001).

Conclusion

Serum miR-15b-3p is elevated in CAS patients. It promotes the proliferation of HA-VSMC cells via targeting PTEN. Although miR-15b-3p holds promise diagnostic value for CAS, it is not yet sufficient for clinical diagnosis.