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<p>Resistant starch (RS) is a dietary component with potential benefits for obesity and metabolic disorders. This study aimed to investigate the anti-adipogenic effects and associated molecular responses of cassava-derived RS type 3 (C-RS3). Thai native cassava starch (C-NS) was modified into C-RS3, and healthy male Wistar rats were fed diets containing C-NS (44.5&#xa0;g%) or C-RS3 (38.1&#xa0;g%) for 28 days. Despite comparable food intake and calorie intake, C-RS3-fed rats exhibited significantly attenuated body weight gain (15.9 ± 2.8%) compared to C-NS-fed rats (34.4 ± 2.6%; <i>p</i> &lt; 0.001). This potent anti-adipogenic effect was confirmed by reduced liver and visceral fat weights, significantly lower serum triglycerides, and decreased hepatic lipid deposition. While overall systemic glucose homeostasis (AUC, fasting glucose, HOMA-IR index, insulin, and glucagon) remained unchanged, C-RS3 significantly lowered the initial glucose peak at 15&#xa0;min post-load during the Oral Glucose Tolerance Test. The core finding of the molecular analysis was the significant elevation of SIRT1 protein in the liver and serum (<i>p</i> &lt; 0.05), despite no change in hepatic <i>Sirt1</i> and <i>Sirt3</i> mRNA levels. Furthermore, correlation analysis revealed a coordinated metabolic and redox-related associations, particularly positive correlation between sirtuin transcripts and antioxidant enzyme transcripts (<i>CAT</i>, <i>GPx</i>, <i>SOD</i>). These findings suggest that C-RS3 attenuates weight gain, adiposity, dyslipidemia, and hepatic lipid accumulation, accompanied by increased SIRT1 protein and coordinated sirtuin–antioxidant-related responses. However, the precise mechanisms underlying these effects require further investigation. C-RS3 may therefore represent a promising functional ingredient for metabolic health management.</p> Graphical Abstract <p></p>

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Impact of cassava-derived resistant starch type 3 on weight gain and hepatic lipid deposition and its association with SIRT1 and antioxidant responses in rats

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月21日

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  • Taratorn Fainanta,
  • Kuakarun Krusong,
  • Watthanachai Saradhuldhat,
  • Thapanan Jatuyosporn,
  • Kanitha Tananuwong,
  • Sukanya Jaroenporn

摘要

Resistant starch (RS) is a dietary component with potential benefits for obesity and metabolic disorders. This study aimed to investigate the anti-adipogenic effects and associated molecular responses of cassava-derived RS type 3 (C-RS3). Thai native cassava starch (C-NS) was modified into C-RS3, and healthy male Wistar rats were fed diets containing C-NS (44.5 g%) or C-RS3 (38.1 g%) for 28 days. Despite comparable food intake and calorie intake, C-RS3-fed rats exhibited significantly attenuated body weight gain (15.9 ± 2.8%) compared to C-NS-fed rats (34.4 ± 2.6%; p < 0.001). This potent anti-adipogenic effect was confirmed by reduced liver and visceral fat weights, significantly lower serum triglycerides, and decreased hepatic lipid deposition. While overall systemic glucose homeostasis (AUC, fasting glucose, HOMA-IR index, insulin, and glucagon) remained unchanged, C-RS3 significantly lowered the initial glucose peak at 15 min post-load during the Oral Glucose Tolerance Test. The core finding of the molecular analysis was the significant elevation of SIRT1 protein in the liver and serum (p < 0.05), despite no change in hepatic Sirt1 and Sirt3 mRNA levels. Furthermore, correlation analysis revealed a coordinated metabolic and redox-related associations, particularly positive correlation between sirtuin transcripts and antioxidant enzyme transcripts (CAT, GPx, SOD). These findings suggest that C-RS3 attenuates weight gain, adiposity, dyslipidemia, and hepatic lipid accumulation, accompanied by increased SIRT1 protein and coordinated sirtuin–antioxidant-related responses. However, the precise mechanisms underlying these effects require further investigation. C-RS3 may therefore represent a promising functional ingredient for metabolic health management.

Graphical Abstract