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<p>Skin wound healing and tissue regeneration remain major global challenges, as even minor or acute wounds can progress into chronic lesions following microbial invasion, involving a complex cascade of biological events and multiple overlapping phases. This study aimed to develop a multifunctional wound dressing patch using cellulose nanofibre (CNF) derived from <i>Musa balbisiana L.</i> using chitosan (CH) and polyvinyl alcohol (PVA) as biopolymers and Au/Pt/Ag nanoparticles as fillers and marigold extract (ME) to enhance its efficacy. The physiochemical characterization of the patches was carried out using Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), and Fourier Transform Infrared spectroscopy (FTIR), and Thermo Gravimetric Analysis (TGA). Functional properties such as swelling, degradation, and hemolysis were also studied. Biomedical evaluations, including antimicrobial assays, in vitro analysis, and in vivo animal studies were performed to assess the ability of the patch as a potential wound healing material. The patch CNF–CH–PVA–Au/Pt/Ag–ME, displayed mechanical strength of 17.00 ± 3.01&#xa0;MPa, flexibility of 25% and swelling percentage as 150.96 ± 9.42%. It also demonstrated biodegradable properties (147.42 ± 9.90%) and high cell viability of up to 110%. Live/dead analysis and scratch assay further confirmed the biocompatibility and proliferation of L929 cells in the presence of patch. Antibacterial studies against four different pathogens were conducted, and highest activity of 34 ± 0.32&#xa0;mm was noted against <i>C. albicans</i>. In vivo experiments carried out with Wistar rats showed higher wound closure rate of the patch treated groups as compared to other groups, including a standard reference formulation. Reduction in both TNF-α (144.5 ± 6.8&#xa0;pg/mL) and IL-1β (161.8 ± 7.0&#xa0;pg/mL) occurred after 21&#xa0;days in the patch-treated group. Furthermore, histological examination revealed that the patch promoted cellular migration, angiogenesis, re-epithelialization, collagen deposition, and granulation tissue formation. Therefore, it can be concluded that the <i>Musa balbisiana L.</i> nanofibre-based patch exhibits significant therapeutic potential for future clinical applications in wound healing.</p>

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Banana Nanofiber-Based Biopolymer Patch with Trimetallic Nanoparticles for Wound Healing

  • 期刊论文

  • 发表日期：

    2026年8月5日

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  • Mridusmita Barman,
  • Bitupan Mohan,
  • Pranami Bharadwaj,
  • Semim Akhtar Ahmed,
  • Devi Basumatary,
  • Debajit Thakur,
  • Jagat Chandra Borah,
  • Rajlakshmi Devi,
  • Devasish Chowdhury

摘要

Skin wound healing and tissue regeneration remain major global challenges, as even minor or acute wounds can progress into chronic lesions following microbial invasion, involving a complex cascade of biological events and multiple overlapping phases. This study aimed to develop a multifunctional wound dressing patch using cellulose nanofibre (CNF) derived from Musa balbisiana L. using chitosan (CH) and polyvinyl alcohol (PVA) as biopolymers and Au/Pt/Ag nanoparticles as fillers and marigold extract (ME) to enhance its efficacy. The physiochemical characterization of the patches was carried out using Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), and Fourier Transform Infrared spectroscopy (FTIR), and Thermo Gravimetric Analysis (TGA). Functional properties such as swelling, degradation, and hemolysis were also studied. Biomedical evaluations, including antimicrobial assays, in vitro analysis, and in vivo animal studies were performed to assess the ability of the patch as a potential wound healing material. The patch CNF–CH–PVA–Au/Pt/Ag–ME, displayed mechanical strength of 17.00 ± 3.01 MPa, flexibility of 25% and swelling percentage as 150.96 ± 9.42%. It also demonstrated biodegradable properties (147.42 ± 9.90%) and high cell viability of up to 110%. Live/dead analysis and scratch assay further confirmed the biocompatibility and proliferation of L929 cells in the presence of patch. Antibacterial studies against four different pathogens were conducted, and highest activity of 34 ± 0.32 mm was noted against C. albicans. In vivo experiments carried out with Wistar rats showed higher wound closure rate of the patch treated groups as compared to other groups, including a standard reference formulation. Reduction in both TNF-α (144.5 ± 6.8 pg/mL) and IL-1β (161.8 ± 7.0 pg/mL) occurred after 21 days in the patch-treated group. Furthermore, histological examination revealed that the patch promoted cellular migration, angiogenesis, re-epithelialization, collagen deposition, and granulation tissue formation. Therefore, it can be concluded that the Musa balbisiana L. nanofibre-based patch exhibits significant therapeutic potential for future clinical applications in wound healing.