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<p>This study reports the green biosynthesis of an Ag/Zn nanocomposite using <i>Ocimum basilicum</i> leaf extract as a natural reducing and stabilizing agent, followed by evaluation of its structural and biological properties. Structural characterization using XRD, FT-IR, FESEM/EDS, DLS, zeta potential, XPS, and BET analyses confirmed the successful formation of crystalline nanoscale Ag/Zn nanocomposite with homogeneous morphology, mesoporous structure, and enhanced colloidal stability. The UV–Vis spectrum had a characteristic absorption behavior characteristic of Ag-based nanostructures, with an interfacial interaction between Ag and Zn phases. The synthesized nanocomposite showed significant concentration dependent antibacterial activity against Gram negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus and Bacillus cereus with inhibition zone of 20 ± 1.27&#xa0;mm, 15 ± 2.41&#xa0;mm and 11 ± 0.66&#xa0;mm respectively. The estimated minimum inhibitory concentrations (MICs) were 0.5, 1 and 5&#xa0;mM, respectively. Cytotoxic evaluation using the MTT assay showed that after 48&#xa0;h exposure it exhibited significant anticancer activity against MCF-7 breast cancer cells with an IC<sub>50</sub> value close to 25&#xa0;µg/mL. The results indicate that the Ag/Zn nanocomposite biosynthesized by the fungus has good biomedical application potential for antimicrobial as well as anticancer therapies.</p>

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Green Biosynthesis of Ag/Zn Nanocomposite Using Ocimum basilicum Leaf Extract: Structural Insights and In-Vitro Antibacterial and Anticancer Activity Against MCF-7 Cells

  • 期刊论文

  • 发表日期：

    2026年7月14日

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  • Salah Hashim Shaheed,
  • Hashim Mohammed Hashim Al Araji,
  • Hadi Sajid Abdulabbas,
  • Yasir Haider Al-Mawlah

摘要

This study reports the green biosynthesis of an Ag/Zn nanocomposite using Ocimum basilicum leaf extract as a natural reducing and stabilizing agent, followed by evaluation of its structural and biological properties. Structural characterization using XRD, FT-IR, FESEM/EDS, DLS, zeta potential, XPS, and BET analyses confirmed the successful formation of crystalline nanoscale Ag/Zn nanocomposite with homogeneous morphology, mesoporous structure, and enhanced colloidal stability. The UV–Vis spectrum had a characteristic absorption behavior characteristic of Ag-based nanostructures, with an interfacial interaction between Ag and Zn phases. The synthesized nanocomposite showed significant concentration dependent antibacterial activity against Gram negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus and Bacillus cereus with inhibition zone of 20 ± 1.27 mm, 15 ± 2.41 mm and 11 ± 0.66 mm respectively. The estimated minimum inhibitory concentrations (MICs) were 0.5, 1 and 5 mM, respectively. Cytotoxic evaluation using the MTT assay showed that after 48 h exposure it exhibited significant anticancer activity against MCF-7 breast cancer cells with an IC50 value close to 25 µg/mL. The results indicate that the Ag/Zn nanocomposite biosynthesized by the fungus has good biomedical application potential for antimicrobial as well as anticancer therapies.