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<p>This research explored the properties of sulfated chitosan obtained from the cuttlebone of <i>Sepia brevimana</i>, with particular focus on its thrombin-inhibiting potential. Chitosan, extracted from cuttlefish and chemically modified through sulfation, was investigated as a possible alternative to conventional anticoagulants such as heparin. To achieve the desired sulfated chitosan, extraction and sulfation processes were carried out, followed by characterization using FTIR spectroscopy, FESEM, and XRD techniques. The anticoagulant activity was evaluated through Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT) assays using sheep plasma. FTIR analysis revealed characteristic peaks at 643&#xa0;cm<sup>−1</sup>, 1159&#xa0;cm<sup>−1</sup>, and 3389&#xa0;cm<sup>−1</sup>, confirming the presence of sulfated groups. FESEM images displayed a flake-like microstructure, while XRD analysis indicated a distinct crystalline pattern, with the most prominent peak observed at 19.53°, suggesting potential biomedical applications. The anticoagulant assays showed clotting times of 130 ± 3.75&#xa0;s for APTT and 76 ± 2.25&#xa0;s for PT, indicating the compound’s enhanced capacity to prolong coagulation compared to chitosan. These findings confirm that sulfated chitosan effectively inhibits thrombin activity and possesses significant anticoagulant properties. Overall, the study suggests that sulfated chitosan derived from cuttlefish waste represents a promising candidate for the development of novel anticoagulant therapies.</p>

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Sulfated Chitosan from Sepia brevimana Cuttlebone: A Marine Biopolymer with Potent Anticoagulant Activity

  • 期刊论文

  • 发表日期：

    2026年5月27日

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  • Hari Vamsh Korukonda,
  • Annathai Pitchai,
  • Pasiyappazham Ramasamy

摘要

This research explored the properties of sulfated chitosan obtained from the cuttlebone of Sepia brevimana, with particular focus on its thrombin-inhibiting potential. Chitosan, extracted from cuttlefish and chemically modified through sulfation, was investigated as a possible alternative to conventional anticoagulants such as heparin. To achieve the desired sulfated chitosan, extraction and sulfation processes were carried out, followed by characterization using FTIR spectroscopy, FESEM, and XRD techniques. The anticoagulant activity was evaluated through Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT) assays using sheep plasma. FTIR analysis revealed characteristic peaks at 643 cm−1, 1159 cm−1, and 3389 cm−1, confirming the presence of sulfated groups. FESEM images displayed a flake-like microstructure, while XRD analysis indicated a distinct crystalline pattern, with the most prominent peak observed at 19.53°, suggesting potential biomedical applications. The anticoagulant assays showed clotting times of 130 ± 3.75 s for APTT and 76 ± 2.25 s for PT, indicating the compound’s enhanced capacity to prolong coagulation compared to chitosan. These findings confirm that sulfated chitosan effectively inhibits thrombin activity and possesses significant anticoagulant properties. Overall, the study suggests that sulfated chitosan derived from cuttlefish waste represents a promising candidate for the development of novel anticoagulant therapies.