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<p>The present project planned to evaluate the effect of <i>Lepidium sativum</i> on the healing of the induced defect of femoral bone in rabbits. Sixteen rabbits of both sexes, were randomly divided into two groups (each group contains 8 rabbits) (control group and treated group); all animals were employed to create femur defect by removing 0.5&#xa0;cm of the femoral bone diaphysis (core) surgically by bone drill under general anesthesia by intramuscular injection of 2%xylazin hydrochloride at dosage (5&#xa0;mg/Kg. B.W.) and 10% ketamine hydrochloride at dosage (35&#xa0;mg/Kg. B.W.) respectively with proper aseptic technique, and Treated group, given orally (daily) of 20&#xa0;g/kg of <i>L. sativum</i> seed extract (20&#xa0;cc aqueous extract). The parameters which were used for evaluation; daily Clinical evaluation, radiographic examination, and histopathological finding for both macro and microscopic examination at the end of 1st, 2nd, 3rd, 4th weeks Post-operation (p. o.), also evaluated the use of aqueous extract of <i>L. sativum</i> seeds as antibacterial substances when used on two types of bacteria <i>Staphylococcus aureus</i> (as an example of a Gram-positive bacteria), <i>Escherichia. coli</i> and <i>Pseudomonus aeruginosa</i> (as an example of a Gram-negative bacteria). The clinical results showed that no infection or complication in site of operation, the inflammatory signs “such as redness and swelling” reduced significantly in the treated group in the 3rd day P.Op. compare with the control group, which the inflammatory signs remain until the 4th day, while the lameness disappears in the treated group at the 3rd day p. o. and at the 4th day p. o. in the control group. The radiographic finding revealed clear callus formation that increase in density and volume in treatment group more than in the control group. The macroscopic examination represents Soft fibrous callus formation at the bone defect which increases in size and hardness, but the treated group seemed more symmetrical than the control group. The histopathological examination showed an increase in new granulation tissue formation which altered to trabecular bone formation. The mature trabecular bone formation changes to lamellae bone formation and a sign of Haversian system in treated group. All these observations were very clear in the treated group, and then the control group, also results of antibacterial proved the ability of the aqueous extract of <i>L. sativum</i> seeds to affect both types of bacteria used in different proportions. It can be concluded that used the <i>L. sativum</i> improve the healing process of bone defect, and can be used as antibacterial substances.</p>

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Therapeutic Potential of Lepidium sativum Extract for Bacterial Infection Control and Bone Healing: In-Vitro and In-Vivo Assessment in Rabbit Model

  • 期刊论文

  • 发表日期：

    2026年7月9日

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  • Ammar M. Hashim,
  • Azhar Jabbar Bohan,
  • Israa A. Hashim,
  • Amal A. Hussein,
  • Majid S. Jabir

摘要

The present project planned to evaluate the effect of Lepidium sativum on the healing of the induced defect of femoral bone in rabbits. Sixteen rabbits of both sexes, were randomly divided into two groups (each group contains 8 rabbits) (control group and treated group); all animals were employed to create femur defect by removing 0.5 cm of the femoral bone diaphysis (core) surgically by bone drill under general anesthesia by intramuscular injection of 2%xylazin hydrochloride at dosage (5 mg/Kg. B.W.) and 10% ketamine hydrochloride at dosage (35 mg/Kg. B.W.) respectively with proper aseptic technique, and Treated group, given orally (daily) of 20 g/kg of L. sativum seed extract (20 cc aqueous extract). The parameters which were used for evaluation; daily Clinical evaluation, radiographic examination, and histopathological finding for both macro and microscopic examination at the end of 1st, 2nd, 3rd, 4th weeks Post-operation (p. o.), also evaluated the use of aqueous extract of L. sativum seeds as antibacterial substances when used on two types of bacteria Staphylococcus aureus (as an example of a Gram-positive bacteria), Escherichia. coli and Pseudomonus aeruginosa (as an example of a Gram-negative bacteria). The clinical results showed that no infection or complication in site of operation, the inflammatory signs “such as redness and swelling” reduced significantly in the treated group in the 3rd day P.Op. compare with the control group, which the inflammatory signs remain until the 4th day, while the lameness disappears in the treated group at the 3rd day p. o. and at the 4th day p. o. in the control group. The radiographic finding revealed clear callus formation that increase in density and volume in treatment group more than in the control group. The macroscopic examination represents Soft fibrous callus formation at the bone defect which increases in size and hardness, but the treated group seemed more symmetrical than the control group. The histopathological examination showed an increase in new granulation tissue formation which altered to trabecular bone formation. The mature trabecular bone formation changes to lamellae bone formation and a sign of Haversian system in treated group. All these observations were very clear in the treated group, and then the control group, also results of antibacterial proved the ability of the aqueous extract of L. sativum seeds to affect both types of bacteria used in different proportions. It can be concluded that used the L. sativum improve the healing process of bone defect, and can be used as antibacterial substances.