<p>In this study, we reported the development of polyelectrolyte complex (PEC) patches based on the biopolymers, xanthan gum (X) &amp;chitosan (C) loaded with pineapple-derived compound bromelain, to enhance wound healing efficiency. Characterization of the patches was carried out through dielectric spectroscopy (DS), zeta potential (ZP), Fourier transform infrared spectroscopy (FTIR) and swelling behavior. Antimicrobial activity was investigated against pathogenic yeast as well as gram-positive and gram-negative bacteria. In vivo wound healing study on albino rats. Histopathological examination of wounded skin was also performed. FTIR and DS confirmed the polyelectrolyte complex (PEC) formation. The incorporation of bromelain has no tangible effect on ZP values, lowers the swelling rate, and increases the permittivity (ε’) more than unloaded counterparts. According to the antimicrobial analysis, incorporation of bromelain in the PEC boosts its antimicrobial efficacy. The biochemical evaluation was performed through measuring tissue activity of matrix Metalloproteinase-9 (MMP-9) as well as tissue levels of hydroxyproline (HP), hexosamine, vascular endothelial growth factor (VEGF), tumor growth factor-β1 (TGF-β1), interleukin-10 (IL-10), interleukin-1β (IL-1β), malondialdehyde (MDA) and reduced glutathione (GSH). Histopathological examination of wounded skin was also performed. Treatment of wounded skin with both X₅₀C₅₀ and Br-loaded X₅₀C₅₀ patches succeeded in ameliorating most of the biochemical changes and histological features in hematoxylin and eosin taken to different groups. The results demonstrated accelerated wound closure, reduced inflammation, and enhanced tissue remodeling compared to control dressings. Br-loaded X₅₀C₅₀ patches have powerful antioxidants and anti-inflammatory effects on wounded skin, which make them promising candidates for wound healing applications.</p> Graphical Abstract <p></p>