Scholarlink

<p>This study evaluates the antibacterial activity of a naturally derived copper oxide-coated calcinated diatomaceous earth (CDE-CuO) formulation against two economically significant plant pathogens: <i>Pectobacterium carotovorum</i> subsp. <i>carotovorum</i> and <i>Pseudomonas syringae</i> pv. <i>syringae</i>. CDE-CuO was prepared by acid-washing and calcining diatomaceous earth (DE), followed by green synthesis of CuO and ultrasonic coating onto the DE surface. This method leverages the inherent porosity and surface chemistry of diatomaceous earth, a naturally occurring siliceous sediment, to enhance nanoparticle dispersion and stability. Energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), and dynamic light scattering (DLS) confirmed uniform Cu deposition, an average hydrodynamic size of 85&#xa0;nm (PDI 0.21), and a zeta potential of − 28 mV, indicating good colloidal stability. Antibacterial assays conducted across a concentration range of 10–10,000&#xa0;mg/L revealed that CDE-CuO achieved 74.4% inhibition of PCC and 76.2% inhibition of PSS at the highest dose after 72&#xa0;h outperforming copper-based protectants (Kocide &amp; Bordeaux mixture). Reactive oxygen species (ROS) assays indicated a 2.1-fold increase in fluorescence intensity in treated cells, suggesting oxidative stress as a key mechanism of action. SEM imaging further revealed extensive morphological damage to bacterial cells exposed to CDE-CuO, supporting its membrane-disruptive properties. Importantly, phytotoxicity tests on tomato and cucumber plants showed no visible damage or significant changes in chlorophyll content (SPAD values: 45.2 ± 1.1 vs. control 44.8 ± 1.3), confirming its plant safety. This work presents CDE-CuO as a naturally sourced and environmentally conscious alternative to conventional copper-based bactericides, offering strong antimicrobial efficacy with minimal ecological footprint.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Copper oxide-coated calcinated diatomaceous earth as a potent agent against plant pathogenic bacteria

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月3日

查看全文
  • Mahdi Salimzadeh,
  • Reza Khakvar,
  • Mahsa Kiyavar,
  • Gholamreza Kiyani,
  • Kadarkarai Murugan

摘要

This study evaluates the antibacterial activity of a naturally derived copper oxide-coated calcinated diatomaceous earth (CDE-CuO) formulation against two economically significant plant pathogens: Pectobacterium carotovorum subsp. carotovorum and Pseudomonas syringae pv. syringae. CDE-CuO was prepared by acid-washing and calcining diatomaceous earth (DE), followed by green synthesis of CuO and ultrasonic coating onto the DE surface. This method leverages the inherent porosity and surface chemistry of diatomaceous earth, a naturally occurring siliceous sediment, to enhance nanoparticle dispersion and stability. Energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), and dynamic light scattering (DLS) confirmed uniform Cu deposition, an average hydrodynamic size of 85 nm (PDI 0.21), and a zeta potential of − 28 mV, indicating good colloidal stability. Antibacterial assays conducted across a concentration range of 10–10,000 mg/L revealed that CDE-CuO achieved 74.4% inhibition of PCC and 76.2% inhibition of PSS at the highest dose after 72 h outperforming copper-based protectants (Kocide & Bordeaux mixture). Reactive oxygen species (ROS) assays indicated a 2.1-fold increase in fluorescence intensity in treated cells, suggesting oxidative stress as a key mechanism of action. SEM imaging further revealed extensive morphological damage to bacterial cells exposed to CDE-CuO, supporting its membrane-disruptive properties. Importantly, phytotoxicity tests on tomato and cucumber plants showed no visible damage or significant changes in chlorophyll content (SPAD values: 45.2 ± 1.1 vs. control 44.8 ± 1.3), confirming its plant safety. This work presents CDE-CuO as a naturally sourced and environmentally conscious alternative to conventional copper-based bactericides, offering strong antimicrobial efficacy with minimal ecological footprint.