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<p>In this study, a simple method to prepare a novel nano adsorbent based on agarose matrix has been built by expanded bed adsorption to obtain the optimum condition by size. Determining the type and number of experiments was designed by Taguchi software and our variables included stirring speed, agarose concentration, nickel dosage, and surfactant percentage. Tow layer Nickel-Agarose Coated immobilized by Cibacron Blue dye ligand (2@NAC-CB) particles have been prepared by the three-phase emulsion method. The results showed that the particle size decreases with increasing stirring speed, despite increasing the initial concentration of the surfactant led to a decrease in the size of the adsorbent particles. However, by increasing the surfactant concentration and stirring speed, the best result for particle size was obtained at 4% surfactant concentration and 950&#xa0;rpm speed. The behavior of agarose-coated adsorbent in different conditions was calculated by measuring the bed expansion coefficient and the axial dispersion coefficient for the slurry phase when measured in a standard coupling. The distribution of particle size and relative quality of adsorbents by keeping the surfactant level constant with the lowest concentration of 0.5% w/v. The size distribution of 2@NAC-CB was less than 200&#xa0;nm. Also, the agarose coating of the metal core has been investigated to produce different geometries in the construction of suitable adsorbents for the purification of numerous protein products. The minimum particle size (optimum) was 50&#xa0;rpm stirring speed, 6% agarose percentage, 0.5&#xa0;mg/ml surfactant concentration, and 2&#xa0;mg nickel dosage. The final result showed that the production of spherical porous adsorbent nanoparticles from natural protein in the above method can be widely used for protein purification in the industry.</p>

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Modeling and preparation of nano adsorbents laminated with agarose for expanded bed adsorption in protein purification

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  • 发表日期：

    2025年8月7日

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  • Roozbeh Mofidian

摘要

In this study, a simple method to prepare a novel nano adsorbent based on agarose matrix has been built by expanded bed adsorption to obtain the optimum condition by size. Determining the type and number of experiments was designed by Taguchi software and our variables included stirring speed, agarose concentration, nickel dosage, and surfactant percentage. Tow layer Nickel-Agarose Coated immobilized by Cibacron Blue dye ligand (2@NAC-CB) particles have been prepared by the three-phase emulsion method. The results showed that the particle size decreases with increasing stirring speed, despite increasing the initial concentration of the surfactant led to a decrease in the size of the adsorbent particles. However, by increasing the surfactant concentration and stirring speed, the best result for particle size was obtained at 4% surfactant concentration and 950 rpm speed. The behavior of agarose-coated adsorbent in different conditions was calculated by measuring the bed expansion coefficient and the axial dispersion coefficient for the slurry phase when measured in a standard coupling. The distribution of particle size and relative quality of adsorbents by keeping the surfactant level constant with the lowest concentration of 0.5% w/v. The size distribution of 2@NAC-CB was less than 200 nm. Also, the agarose coating of the metal core has been investigated to produce different geometries in the construction of suitable adsorbents for the purification of numerous protein products. The minimum particle size (optimum) was 50 rpm stirring speed, 6% agarose percentage, 0.5 mg/ml surfactant concentration, and 2 mg nickel dosage. The final result showed that the production of spherical porous adsorbent nanoparticles from natural protein in the above method can be widely used for protein purification in the industry.