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<p>Nosocomial infections are a major concern worldwide. Currently, addressing this issue presents several challenges due to the increasing rise of microbial antibiotic resistance. The present study isolated and identified multidrug-resistant <i>Escherichia coli</i> MED10, which is compatible with nosocomial pathogens in humans, using 16&#xa0;S rRNA gene sequencing. Myco-synthesized selenium nanoparticles (Se NPs) produced by <i>Fusarium fujikuroi</i> MED14 were characterized through ultraviolet-visible spectroscopy, X-ray diffraction (XRD) pattern, Fourier transform infrared (FTIR) spectroscopy, Zeta potential, and transmission electron microscopy (TEM) analyses. Structural characterization of Se NPs revealed spherical shapes measuring 10–19&#xa0;nm, along with a broad absorption peak at 240–270&#xa0;nm. XRD indicated that the crystal planes were 100, 101, 222, and 102. FTIR confirmed the presence of proteins, as well as amide I, amide II, and amide III, corresponding to the peaks observed at 2358&#xa0;cm-1, 1645&#xa0;cm-1, 1566&#xa0;cm-1, and 1254&#xa0;cm-1, respectively. The measured zeta potential of Se NPs was − 43 mV, indicating a highly negative surface charge that reflects their excellent colloidal stability and resistance to aggregation. Antibacterial effects of Se NPs against <i>E. coli</i> MED10, <i>E. coli</i> ATCC 25,922, and <i>Bacillus cereus</i> ATCC 14,579 were examined using the minimum inhibition concentration test (MIC values of 160, 100, and 90&#xa0;µg/ml, respectively) as well as the lowest bactericidal concentration test (MBC values of 170, 110, and 90&#xa0;µg/ml, respectively). Ultrastructural studies on Se NPs-treated <i>E. coli</i> MED10 cells revealed wrinkled and damaged rod shapes, with detachment observed between the cell wall and plasma membrane compared to untreated cells. This investigation reported that myco-synthesized Se NPs, coated with crude metabolites of <i>F. fujikuroi</i> MED14, may act as a synergistic system to help control multidrug-resistant bacteria, including nosocomial <i>E. coli</i> MED10.</p>

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In vitro antibacterial activity of myco-synthesized selenium nanoparticles against a multidrug-resistant E. coli isolated from clinical specimens

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月15日

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  • Mayada F. El-Fawal,
  • Amira A. El-Fallal,
  • Mohamed I. Abou-Dobara,
  • Ahmed K. A. El-Sayed,
  • Mohamed M. El-Zahed

摘要

Nosocomial infections are a major concern worldwide. Currently, addressing this issue presents several challenges due to the increasing rise of microbial antibiotic resistance. The present study isolated and identified multidrug-resistant Escherichia coli MED10, which is compatible with nosocomial pathogens in humans, using 16 S rRNA gene sequencing. Myco-synthesized selenium nanoparticles (Se NPs) produced by Fusarium fujikuroi MED14 were characterized through ultraviolet-visible spectroscopy, X-ray diffraction (XRD) pattern, Fourier transform infrared (FTIR) spectroscopy, Zeta potential, and transmission electron microscopy (TEM) analyses. Structural characterization of Se NPs revealed spherical shapes measuring 10–19 nm, along with a broad absorption peak at 240–270 nm. XRD indicated that the crystal planes were 100, 101, 222, and 102. FTIR confirmed the presence of proteins, as well as amide I, amide II, and amide III, corresponding to the peaks observed at 2358 cm-1, 1645 cm-1, 1566 cm-1, and 1254 cm-1, respectively. The measured zeta potential of Se NPs was − 43 mV, indicating a highly negative surface charge that reflects their excellent colloidal stability and resistance to aggregation. Antibacterial effects of Se NPs against E. coli MED10, E. coli ATCC 25,922, and Bacillus cereus ATCC 14,579 were examined using the minimum inhibition concentration test (MIC values of 160, 100, and 90 µg/ml, respectively) as well as the lowest bactericidal concentration test (MBC values of 170, 110, and 90 µg/ml, respectively). Ultrastructural studies on Se NPs-treated E. coli MED10 cells revealed wrinkled and damaged rod shapes, with detachment observed between the cell wall and plasma membrane compared to untreated cells. This investigation reported that myco-synthesized Se NPs, coated with crude metabolites of F. fujikuroi MED14, may act as a synergistic system to help control multidrug-resistant bacteria, including nosocomial E. coli MED10.