Scholarlink

<p>Handheld devices have become popular to determine epidermal contents of UV-A absorbing compounds in intact leaves. For the comparability of the results between different labs, it is important that all instruments show the same or at least similar results. Therefore, the signals from seven different Dualex Scientific optical leaf clips from five laboratories were compared to each other, alongside a prototype of an LSA-2050 leaf state analyzer and a UV-A-PAM fluorometer. The values of the Flav index, which is used to quantify epidermal UV-A absorbing compounds such as flavonoids, corresponded well with each other (r<sup>2</sup> &gt; 0.989), except for one instrument. A higher variability among instruments was found for the chlorophyll (Chl) index, again with one instrument showing a significant offset. Although one Dualex instrument showed an excellent linear calibration for the anthocyanin (Anth) index relative to extract concentrations when red hazelnut leaves were investigated (r<sup>2</sup> = 0.969), the Dualex instruments had all an offset against each other and at Chl index values below 30, erroneous Anth index values were observed. While Flav index results appeared quite reliable, it is recommended to calibrate a Dualex instrument against leaf extracts for exact determination of chlorophyll and anthocyanin contents. Simple tests for the functionality of a Dualex instrument are suggested.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Optical detection of pigment contents in leaves: intercomparison of several handheld devices

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月4日

查看全文
  • Wolfgang Bilger,
  • Gyula Czégény,
  • Susanne Neugart,
  • Jakub Nezval,
  • Erhard Pfündel,
  • Adriana Volná

摘要

Handheld devices have become popular to determine epidermal contents of UV-A absorbing compounds in intact leaves. For the comparability of the results between different labs, it is important that all instruments show the same or at least similar results. Therefore, the signals from seven different Dualex Scientific optical leaf clips from five laboratories were compared to each other, alongside a prototype of an LSA-2050 leaf state analyzer and a UV-A-PAM fluorometer. The values of the Flav index, which is used to quantify epidermal UV-A absorbing compounds such as flavonoids, corresponded well with each other (r2 > 0.989), except for one instrument. A higher variability among instruments was found for the chlorophyll (Chl) index, again with one instrument showing a significant offset. Although one Dualex instrument showed an excellent linear calibration for the anthocyanin (Anth) index relative to extract concentrations when red hazelnut leaves were investigated (r2 = 0.969), the Dualex instruments had all an offset against each other and at Chl index values below 30, erroneous Anth index values were observed. While Flav index results appeared quite reliable, it is recommended to calibrate a Dualex instrument against leaf extracts for exact determination of chlorophyll and anthocyanin contents. Simple tests for the functionality of a Dualex instrument are suggested.