Scholarlink

<p>Survival of organisms depends on their ability to adapt to their environment. Urbanization is increasing rapidly, resulting in elevated levels of artificial night illumination. A wide range of organisms may be affected by this global change in the urban environment. This study examined the effect of exposure to artificial light (0.0276&#xa0;W/m<sup>2</sup>) at night (ALAN) on growth, development, and transcript expression of <i>Bombyx mori</i>, a common but economically important moth. The newly hatched <i>B. mori</i> larvae were randomly divided into two groups. Both groups were exposed to a daily light–dark cycle of 12L:12D (12&#xa0;h light and 12&#xa0;h dark), while group 1 served as the control. In group 2, the dark phase was illuminated with dim light at 0.0276&#xa0;W/m<sup>2</sup>. Cocoon quality parameters, including cocoon weight, cocoon shell weight, cocoon shell ratio, pupal weight, and fertilization ratio, were studied. Daily expression of clock genes (<i>Per</i>, <i>Cry1</i>, and <i>Tim</i>), transcripts involved in hormone synthesis (<i>Nvd</i>, <i>Dib</i>, <i>Phm</i>, <i>Spo</i>, <i>Jhamt</i>, and <i>Ptth</i>), and 20E-inducible transcripts (<i>Hr3</i>, <i>Hr4</i>, <i>E75A</i>, and <i>Bftz</i>) were measured. Results suggest that exposure to ALAN affects cocoon parameters, downregulates the daily rhythmic expression of <i>Tim</i>, and leads to a loss of rhythmicity in the expression of clock gene transcripts <i>Cry1</i> and <i>Per</i>. Further, downregulation of hormone transcripts (<i>Nvd</i>, <i>Dib</i>, <i>Phm</i>, <i>Spo</i>, <i>Hr3</i>, <i>E75A</i>, <i>Jhamt</i>, and <i>Ptth</i>) was also observed in the treated group. These results are important, as they suggest that exposure to ALAN compromises silk moth development under laboratory conditions and may affect overall silk production/quality.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Artificial light at night (ALAN) negatively affects expression of transcripts involved in biosynthesis of molting and juvenile hormones in Bombyx mori

  • 期刊论文

  • 发表日期：

    2026年1月19日

查看全文
  • Zothanmawii Renthlei,
  • Tlau S. K. Lalrinawma,
  • Lalsiamkima Hnamler,
  • Shalie Malik,
  • Amit Kumar Trivedi

摘要

Survival of organisms depends on their ability to adapt to their environment. Urbanization is increasing rapidly, resulting in elevated levels of artificial night illumination. A wide range of organisms may be affected by this global change in the urban environment. This study examined the effect of exposure to artificial light (0.0276 W/m2) at night (ALAN) on growth, development, and transcript expression of Bombyx mori, a common but economically important moth. The newly hatched B. mori larvae were randomly divided into two groups. Both groups were exposed to a daily light–dark cycle of 12L:12D (12 h light and 12 h dark), while group 1 served as the control. In group 2, the dark phase was illuminated with dim light at 0.0276 W/m2. Cocoon quality parameters, including cocoon weight, cocoon shell weight, cocoon shell ratio, pupal weight, and fertilization ratio, were studied. Daily expression of clock genes (Per, Cry1, and Tim), transcripts involved in hormone synthesis (Nvd, Dib, Phm, Spo, Jhamt, and Ptth), and 20E-inducible transcripts (Hr3, Hr4, E75A, and Bftz) were measured. Results suggest that exposure to ALAN affects cocoon parameters, downregulates the daily rhythmic expression of Tim, and leads to a loss of rhythmicity in the expression of clock gene transcripts Cry1 and Per. Further, downregulation of hormone transcripts (Nvd, Dib, Phm, Spo, Hr3, E75A, Jhamt, and Ptth) was also observed in the treated group. These results are important, as they suggest that exposure to ALAN compromises silk moth development under laboratory conditions and may affect overall silk production/quality.