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<p>The present study was carried out to assess phytochemical composition and antimicrobial activity of aqueous leaf extracts from <i>Callistemon viminalis</i> and <i>Murraya koenigii</i> collected from Dehradun, Uttarakhand. Both the extract was prepared using hot water maceration and then spectrophotometric analysis of extracts revealed that the <i>M. koenigii</i> contained 1.08% alkaloids, 0.28% flavonoids, 1.22% phenols, and 0.76% terpenoids, while the <i>C. viminalis</i> exhibited 0.86% alkaloids, 0.11% flavonoids, 2.89% phenols, and 1.02% terpenoids. Antimicrobial assays (nutrient agar and sabouraud dextrose agar) demonstrated no inhibition against tested bacteria <i>(Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa)</i> or <i>fungi (Aspergillus niger, Candida albicans).</i> The absence of bioactivity in aqueous extracts underscores the needs to explore alternative solvents and fractionation strategies for isolating bioactive compounds. The study highlights the limitations of aqueous extraction for antimicrobial applications, and the presence of several phytochemicals points to possible bioactivity that calls for further research using compound isolation, alternate extraction techniques, and more extensive biological screening.</p>

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Phytochemical and antimicrobial profile of aqueous leaf extracts of Callistemon viminalis and Murraya koenigii from Dehradun

  • 期刊论文

  • 发表日期：

    2026年7月10日

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  • Deepti Singh Vashishth,
  • Rinky Yadav,
  • Rakesh Kumar Bachheti,
  • Ashish Kumar Arya,
  • Archana Bachheti

摘要

The present study was carried out to assess phytochemical composition and antimicrobial activity of aqueous leaf extracts from Callistemon viminalis and Murraya koenigii collected from Dehradun, Uttarakhand. Both the extract was prepared using hot water maceration and then spectrophotometric analysis of extracts revealed that the M. koenigii contained 1.08% alkaloids, 0.28% flavonoids, 1.22% phenols, and 0.76% terpenoids, while the C. viminalis exhibited 0.86% alkaloids, 0.11% flavonoids, 2.89% phenols, and 1.02% terpenoids. Antimicrobial assays (nutrient agar and sabouraud dextrose agar) demonstrated no inhibition against tested bacteria (Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa) or fungi (Aspergillus niger, Candida albicans). The absence of bioactivity in aqueous extracts underscores the needs to explore alternative solvents and fractionation strategies for isolating bioactive compounds. The study highlights the limitations of aqueous extraction for antimicrobial applications, and the presence of several phytochemicals points to possible bioactivity that calls for further research using compound isolation, alternate extraction techniques, and more extensive biological screening.