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<p><?tk 2?><i>Bergenia ligulata</i> (Wall.) Engl. is recognized for its diverse medicinal properties, but its immunomodulatory effects remain underexplored. This study examines the antioxidative, immunostimulatory and anti-inflammatory efficacy of aqueous rhizome extract of <i>Bergenia ligulata</i> (BLE) using chicken splenocytes culture system- an approach not previously explored for <i>Bergenia</i> spp. BLE was analyzed using GC–MS, LC–MS, in vitro antioxidant and anti-inflammatory analyses. Its maximum non-cytotoxic dose (MNCD) in chicken splenocytes was determined through colorimetric- MTT assay. Dexamethasone (DEXA), a known corticosteroid, was used as a control in the study. BLE revealed presence of various phytochemicals through GC–MS, LC–MS analyses and significant antioxidant potential in different in vitro assays conducted. BLE showed MNCD as 200 µg/mL. BLE displayed increased lymphocyte proliferation upon mitogenic stimulation (immunopotentiating effect), whereas DEXA exhibited immunosuppressive effects. BLE exposure downregulated the pro-inflammatory mediators while upregulating the expression of IL-10 which is an important anti-inflammatory cytokine, demonstrating its potent anti-inflammatory properties. Additionally, BLE exposure up-regulated Nrf-2 level, indicating its ability to combat oxidative stress. BLE exposure significantly increased immunostimulatory mediators (NFAT-1 and -2), while DEXA caused reduction in the expression levels of these transcription factors and cytokines. In all, BLE exhibited presence of various phyochemicals and significant in vitro antioxidant, immunopotentiating and anti-inflammatory bioactivities. The findings underscore the multifaceted anti-inflammatory, immunostimulatory and antioxidant effects of BLE on chicken splenocytes, shedding light on its therapeutic potential in acute- inflammatory animal cell-culture model system induced by LPS-stimulation in chicken-splenocytes.</p> Graphical abstract <p></p>

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Phyto-profiling of Bergenia ligulata rhizome extract and it’s in vitro antioxidative, anti-inflammatory and immunomodulatory potential: mechanistic insights from differential gene expression in acute inflammatory model of chicken splenocytes

  • 期刊论文

  • 发表日期：

    2026年7月4日

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  • Sonu Ambwani,
  • Shivani

摘要

Bergenia ligulata (Wall.) Engl. is recognized for its diverse medicinal properties, but its immunomodulatory effects remain underexplored. This study examines the antioxidative, immunostimulatory and anti-inflammatory efficacy of aqueous rhizome extract of Bergenia ligulata (BLE) using chicken splenocytes culture system- an approach not previously explored for Bergenia spp. BLE was analyzed using GC–MS, LC–MS, in vitro antioxidant and anti-inflammatory analyses. Its maximum non-cytotoxic dose (MNCD) in chicken splenocytes was determined through colorimetric- MTT assay. Dexamethasone (DEXA), a known corticosteroid, was used as a control in the study. BLE revealed presence of various phytochemicals through GC–MS, LC–MS analyses and significant antioxidant potential in different in vitro assays conducted. BLE showed MNCD as 200 µg/mL. BLE displayed increased lymphocyte proliferation upon mitogenic stimulation (immunopotentiating effect), whereas DEXA exhibited immunosuppressive effects. BLE exposure downregulated the pro-inflammatory mediators while upregulating the expression of IL-10 which is an important anti-inflammatory cytokine, demonstrating its potent anti-inflammatory properties. Additionally, BLE exposure up-regulated Nrf-2 level, indicating its ability to combat oxidative stress. BLE exposure significantly increased immunostimulatory mediators (NFAT-1 and -2), while DEXA caused reduction in the expression levels of these transcription factors and cytokines. In all, BLE exhibited presence of various phyochemicals and significant in vitro antioxidant, immunopotentiating and anti-inflammatory bioactivities. The findings underscore the multifaceted anti-inflammatory, immunostimulatory and antioxidant effects of BLE on chicken splenocytes, shedding light on its therapeutic potential in acute- inflammatory animal cell-culture model system induced by LPS-stimulation in chicken-splenocytes.

Graphical abstract