<p>Maternal environment plays a vital role in shaping morphological variability in invasive plants, which can enhance their ecological adaptability under heterogeneous environmental conditions. Therefore, the present study was assessed to examine the influence of population origin on structural, functional, and reproductive characteristics of an invasive plant Crofton weed (<i>Ageratina adenophora</i>). Seeds were collected from eight geographically different populations of Uttarakhand, India, and seedlings were grown under uniform greenhouse conditions to minimize environmental variations during the plant developmental phases. Multiple structural, functional, and reproductive traits were measured, and differences among populations were evaluated using analysis of variance (ANOVA) and Principal Component Analysis (PCA). Results indicated that most primary structural traits, including shoot length, root length, and leaf morphological characteristics, did not vary statistically among populations, suggesting morphological plasticity among different populations when grown under uniform environmental conditions. In contrast, several functional and reproductive traits related to allocation and seed production showed significant population level variations demonstrating strong maternal or epigenetic influences on resource allocation and reproductive investment. Because all plants were grown under identical environmental conditions, the observed differences primarily reflect maternal effects, transgenerational plasticity, or epigenetic variations. The results suggest role of maternal origin in influencing allocation strategies and reproductive performance more strongly than basic plant size traits and high degree of morphological plasticity in plant structural traits. Such variability may contribute to the ecological flexibility and invasion success of <i>A. adenophora</i> by enabling populations to produce offspring with diverse functional strategies across varying environments.</p>