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<p><i>Candida tropicalis</i> is an opportunistic fungal pathogen known for its strong biofilm-forming ability, which contributes to increased resistance against antifungal treatments and complicates infection management, particularly in immunocompromised individuals. This study investigates the efficacy of rhamnolipid biosurfactants derived from <i>Pseudomonas chlororaphis</i> in preventing and disrupting <i>C. tropicalis</i> biofilms. The surface-active properties of these biosurfactants were analyzed to determine their ability to interfere with biofilm formation. The biofilm and fungal-inhibitory effects were assessed in the current study using crystal violet assays, disk diffusion assays, and scanning electron microscopy (SEM), which demonstrated a significant reduction in biofilm biomass and structural integrity upon treatment with biosurfactant. Additionally, molecular docking studies were carried out which provided insights into the interactions between rhamnolipids and biofilm-associated proteins, suggesting a potential mechanism for biofilm disruption. To explore practical applications, a rhamnolipid-based cleanser was formulated and tested for its antifungal efficacy. The cleanser effectively disrupted preformed <i>C. tropicalis</i> biofilms and inhibited new biofilm formation, highlighting its potential use in medical and industrial settings. These findings support the application of rhamnolipid biosurfactants as a sustainable and effective strategy for controlling fungal biofilms, particularly in healthcare environments where biofilm-related infections pose a significant challenge.</p>

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Eco-friendly biofilm disruption: rhamnolipids as a novel antifungal agent

  • 期刊论文

  • 发表日期：

    2026年1月24日

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  • Prerana Rao,
  • Ramya Rajan,
  • Varsha Raghavendra,
  • Angira Devi Bhuyan

摘要

Candida tropicalis is an opportunistic fungal pathogen known for its strong biofilm-forming ability, which contributes to increased resistance against antifungal treatments and complicates infection management, particularly in immunocompromised individuals. This study investigates the efficacy of rhamnolipid biosurfactants derived from Pseudomonas chlororaphis in preventing and disrupting C. tropicalis biofilms. The surface-active properties of these biosurfactants were analyzed to determine their ability to interfere with biofilm formation. The biofilm and fungal-inhibitory effects were assessed in the current study using crystal violet assays, disk diffusion assays, and scanning electron microscopy (SEM), which demonstrated a significant reduction in biofilm biomass and structural integrity upon treatment with biosurfactant. Additionally, molecular docking studies were carried out which provided insights into the interactions between rhamnolipids and biofilm-associated proteins, suggesting a potential mechanism for biofilm disruption. To explore practical applications, a rhamnolipid-based cleanser was formulated and tested for its antifungal efficacy. The cleanser effectively disrupted preformed C. tropicalis biofilms and inhibited new biofilm formation, highlighting its potential use in medical and industrial settings. These findings support the application of rhamnolipid biosurfactants as a sustainable and effective strategy for controlling fungal biofilms, particularly in healthcare environments where biofilm-related infections pose a significant challenge.