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<p>Gas Chromatography (GC), Gas Chromatography-Mass Spectrometry (GC–MS), and High-Performance Liquid Chromatography (HPLC) represent fundamental analytical techniques in fisheries research, particularly for the detection, separation, and quantification of various contaminants and biochemical compounds. GC is primarily employed for the analysis of volatile organic compounds, while the coupling of GC with mass spectrometry (GC–MS) enhances specificity and sensitivity, allowing for the precise identification of trace organic pollutants. In contrast, HPLC is better suited for the analysis of non-volatile, polar, or thermally unstable compounds, such as antibiotics, proteins, and hormones. This review conducts a comprehensive comparative analysis of the applications of GC, GC–MS, and HPLC in fisheries, with a focus on their current utilization in environmental monitoring, food safety, and fish toxicology. Furthermore, it evaluates their limitations and offers insights into future advancements, including automation, miniaturization, and artificial intelligence-driven data analysis. By exploring these developments, this review provides a robust framework for selecting the most appropriate chromatographic method for specific research applications, ultimately underscoring the significance of these technologies in advancing sustainable fisheries management and environmental protection.</p>

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Understanding applications of chromatographic techniques in fisheries: present status and future perspective

  • 期刊论文

  • 发表日期：

    2025年3月15日

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  • Farheen Saba,
  • Imtiyaz Qayoom,
  • Masroor Fatima,
  • Adnan Abubakr,
  • Sameena Khan,
  • Taranum Mushtaq,
  • Fazle Rasool,
  • Mehjabeena Akhter

摘要

Gas Chromatography (GC), Gas Chromatography-Mass Spectrometry (GC–MS), and High-Performance Liquid Chromatography (HPLC) represent fundamental analytical techniques in fisheries research, particularly for the detection, separation, and quantification of various contaminants and biochemical compounds. GC is primarily employed for the analysis of volatile organic compounds, while the coupling of GC with mass spectrometry (GC–MS) enhances specificity and sensitivity, allowing for the precise identification of trace organic pollutants. In contrast, HPLC is better suited for the analysis of non-volatile, polar, or thermally unstable compounds, such as antibiotics, proteins, and hormones. This review conducts a comprehensive comparative analysis of the applications of GC, GC–MS, and HPLC in fisheries, with a focus on their current utilization in environmental monitoring, food safety, and fish toxicology. Furthermore, it evaluates their limitations and offers insights into future advancements, including automation, miniaturization, and artificial intelligence-driven data analysis. By exploring these developments, this review provides a robust framework for selecting the most appropriate chromatographic method for specific research applications, ultimately underscoring the significance of these technologies in advancing sustainable fisheries management and environmental protection.