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Background <p>Osteoporosis is a major global health concern, increasing the risk of fragility fractures, morbidity, and mortality. Conventional diagnostic tools such as dual-energy X-ray absorptiometry (DXA) have limitations in accurately predicting fracture risk. Recent studies suggest that microRNAs (miRNAs) play a crucial role in bone metabolism and may serve as potential biomarkers for osteoporosis and fracture susceptibility.</p> Objective <p>This study aimed to identify differentially expressed miRNAs in osteoporotic and non-osteoporotic trauma patients with proximal femur fractures and assess their clinical significance as diagnostic and prognostic biomarkers.</p> Methods <p>A prospective case–control study was conducted on 20 patients (10 osteoporotic, 10 non-osteoporotic) with proximal femur fractures. Serum biochemical markers [vitamin D, and intact parathyroid hormone (iPTH)], were analyzed. miRNA profiling was performed using the GeneChip® miRNA 4.0 Array to identify differentially expressed miRNAs. Statistical analysis included fold change expression, hierarchical clustering, and false discovery rate (FDR) correction.</p> Results <p>Osteoporotic patients (Group I) exhibited significantly lower vitamin D3 levels (15.8–23.1&#xa0;ng/mL) and iPTH levels (9.6–16.4&#xa0;pg/mL) compared to non-osteoporotic individuals (Group II), who had higher vitamin D3 (24.2–46.8&#xa0;ng/mL) and iPTH levels (15.6–28.9&#xa0;pg/mL). DXA scans confirmed severe bone loss in osteoporotic patients (T-score: −3.9 to −4.6), while non-osteoporotic individuals had normal bone density (T-score: −0.5 to −0.9). miRNA expression analysis revealed significant downregulation of Four miRNAs (miR-320b, miR-320c, miR-486-5p, and let-7b-5p) in osteoporotic patients. miR-320b and miR-320c have been previously associated with osteogenic differentiation, while miR-let-7b plays a role in inflammation and bone formation. These findings suggest a potential regulatory role of these miRNAs in osteoporosis pathogenesis.</p> Conclusion <p>This study highlights miR-320b, miR-320c, and miR-let-7b as potential biomarkers for osteoporosis and fracture susceptibility. The identification of distinct miRNA signatures in osteoporotic fractures suggests that integrating miRNA profiling with biochemical and imaging assessments could enhance early diagnosis and personalized fracture risk prediction. These findings pave the way for innovative diagnostic and therapeutic strategies in osteoporosis management, ultimately improving fracture prevention in high-risk individuals. Further large-scale studies are warranted to validate these biomarkers for clinical application.</p>

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Differential MicroRNA Expression in Osteoporotic and Non-Osteoporotic Trauma Patients: A Potential Biomarker Approach for Fracture Risk Assessment

  • 期刊论文

  • 发表日期：

    2026年1月7日

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  • Venencia Albert,
  • Arulselvi Subramanian,
  • Shyam Prakash,
  • Vivek Trikha,
  • Anand Kumar,
  • Rajesh Malhotra

摘要

Background

Osteoporosis is a major global health concern, increasing the risk of fragility fractures, morbidity, and mortality. Conventional diagnostic tools such as dual-energy X-ray absorptiometry (DXA) have limitations in accurately predicting fracture risk. Recent studies suggest that microRNAs (miRNAs) play a crucial role in bone metabolism and may serve as potential biomarkers for osteoporosis and fracture susceptibility.

Objective

This study aimed to identify differentially expressed miRNAs in osteoporotic and non-osteoporotic trauma patients with proximal femur fractures and assess their clinical significance as diagnostic and prognostic biomarkers.

Methods

A prospective case–control study was conducted on 20 patients (10 osteoporotic, 10 non-osteoporotic) with proximal femur fractures. Serum biochemical markers [vitamin D, and intact parathyroid hormone (iPTH)], were analyzed. miRNA profiling was performed using the GeneChip® miRNA 4.0 Array to identify differentially expressed miRNAs. Statistical analysis included fold change expression, hierarchical clustering, and false discovery rate (FDR) correction.

Results

Osteoporotic patients (Group I) exhibited significantly lower vitamin D3 levels (15.8–23.1 ng/mL) and iPTH levels (9.6–16.4 pg/mL) compared to non-osteoporotic individuals (Group II), who had higher vitamin D3 (24.2–46.8 ng/mL) and iPTH levels (15.6–28.9 pg/mL). DXA scans confirmed severe bone loss in osteoporotic patients (T-score: −3.9 to −4.6), while non-osteoporotic individuals had normal bone density (T-score: −0.5 to −0.9). miRNA expression analysis revealed significant downregulation of Four miRNAs (miR-320b, miR-320c, miR-486-5p, and let-7b-5p) in osteoporotic patients. miR-320b and miR-320c have been previously associated with osteogenic differentiation, while miR-let-7b plays a role in inflammation and bone formation. These findings suggest a potential regulatory role of these miRNAs in osteoporosis pathogenesis.

Conclusion

This study highlights miR-320b, miR-320c, and miR-let-7b as potential biomarkers for osteoporosis and fracture susceptibility. The identification of distinct miRNA signatures in osteoporotic fractures suggests that integrating miRNA profiling with biochemical and imaging assessments could enhance early diagnosis and personalized fracture risk prediction. These findings pave the way for innovative diagnostic and therapeutic strategies in osteoporosis management, ultimately improving fracture prevention in high-risk individuals. Further large-scale studies are warranted to validate these biomarkers for clinical application.