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<p>In this study, we aimed to clarify the effect of icariin on spermatogonia proliferation and the underlying mechanisms. Mouse spermatogonia were treated with different concentrations of icariin, followed by treatment with apalutamide (androgen receptor inhibitor) or tamoxifen (estrogen receptor inhibitor). Immunocytochemistry was performed to assess cell proliferation and the activation of androgen and estrogen signal pathways. Icariin elevated 5-bromo-2′-deoxyuridine, proliferating cell nuclear antigen, synaptonemal complex protein 3, meiotic recombination protein 8, specificity protein-1, cAMP-response element binding protein, and calcium-calmodulin-dependent protein kinase II expression levels in spermatogonia in a dose-dependent manner. Both apalutamide and tamoxifen reduced the increased 5-bromo-2′-deoxyuridine, proliferating cell nuclear antigen, synaptonemal complex protein 3, and meiotic recombination protein 8 induced by icariin in spermatogonia. These results suggested that icariin promoted spermatogonia proliferation via mitosis by activating androgen and estrogen signal pathways <i>in vitro</i>.</p> Graphical Abstract <p></p>

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Icariin Promotes Spermatogonia Proliferation via Regulating Androgen and Estrogen Signaling Pathways In Vitro

  • 期刊论文

  • 发表日期：

    2025年12月5日

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  • Yuji Jin,
  • Haiying Shen,
  • Cheng Wang,
  • Weijian Liu,
  • Cheng Wen,
  • Xinrui Zheng,
  • Zhixiong Li,
  • Yunjian Chen,
  • Yingji Jin,
  • Yong Jiang

摘要

In this study, we aimed to clarify the effect of icariin on spermatogonia proliferation and the underlying mechanisms. Mouse spermatogonia were treated with different concentrations of icariin, followed by treatment with apalutamide (androgen receptor inhibitor) or tamoxifen (estrogen receptor inhibitor). Immunocytochemistry was performed to assess cell proliferation and the activation of androgen and estrogen signal pathways. Icariin elevated 5-bromo-2′-deoxyuridine, proliferating cell nuclear antigen, synaptonemal complex protein 3, meiotic recombination protein 8, specificity protein-1, cAMP-response element binding protein, and calcium-calmodulin-dependent protein kinase II expression levels in spermatogonia in a dose-dependent manner. Both apalutamide and tamoxifen reduced the increased 5-bromo-2′-deoxyuridine, proliferating cell nuclear antigen, synaptonemal complex protein 3, and meiotic recombination protein 8 induced by icariin in spermatogonia. These results suggested that icariin promoted spermatogonia proliferation via mitosis by activating androgen and estrogen signal pathways in vitro.

Graphical Abstract