Scholarlink

<p><i>Fridericia</i><i> chica</i> (Bonpl.) L.G.Lohmann, Bignoniaceae, is popularly used to treat gastrointestinal disorders. This study aimed to evaluate the antiulcer activities of a hydroalcoholic extract of <i>F. chica</i> and to advance the understanding of the pharmacological mechanisms through <i>in silico</i>, <i>in vivo</i>, and <i>ex vivo</i> studies. For the gastric healing evaluation, rats were induced with acetic acid (80%) and received the following treatments orally for 7&#xa0;days, twice a day: vehicle (1&#xa0;ml/kg), omeprazole (20&#xa0;mg/kg), or the hydroalcoholic extract (30&#xa0;mg/kg). The area of the lesion was assessed, and the healing quality was also measured by ultrasonographic analyses. Ulcer recurrence was evaluated in mice induced with interleukin (IL)-1β (1&#xa0;μg/kg, <i>i.p</i>.). Furthermore, measurements were taken for levels of reduced glutathione (GSH) and malondialdehyde, as well as the activity of superoxide dismutase, catalase, glutathione <i>S</i>-transferase, <i>N</i>-acetyl-β-<span>d</span>-glycosaminidase, and myeloperoxidase. Moreover, histological and phytochemical analyses were performed. The hydroalcoholic extract accelerated gastric healing by 69.2% and prevented its recurrence by 94%. These findings were observed by histological, biochemical, and ultrasound assays, which showed mucosal preservation. Additionally, the gastric healing of the hydroalcoholic extract caused a reduction in myeloperoxidase and increased catalase activities. In addition, the reduced glutathione levels and <i>N</i>-acetyl-β-<span>d</span>-glycosaminidase activity were restored in the recurrence test. <i>In silico</i>, compounds from the hydroalcoholic extract, characterized by mass spectrometry (ESI-IT-MS<sup>n</sup>), revealed pathways associated with oxidative stress and inflammatory processes. The hydroalcoholic extract exhibits antiulcer effects attributed essentially to flavonoids, which demonstrate mucosal protective, anti-inflammatory, and antioxidant properties, without evidence of cytotoxicity.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Integration of In Vivo and In Silico Studies Reveals that the Hydroalcoholic Extract of Fridericia chica Improves Gastric Healing and Reduces Recurrence in Rodents

  • 期刊论文

  • 发表日期：

    2025年10月13日

查看全文
  • Daniela Miorando,
  • Amanda M. Steffler,
  • Viviane L. Simomura,
  • Cristian A. Dalla Vecchia,
  • Cleidiane V. Ferraz,
  • Juliana C. Maccagnan,
  • Aline Mânica,
  • Jaqueline J. Veloso,
  • Maike V. Buzatto,
  • André L. Von Dentz,
  • Giovana T. Capoani,
  • Vanessa C. Schemmer,
  • Lincon B. Somensi,
  • Larissa Venzon,
  • Thiago F. de Queiroz eSilva,
  • Max Vidal Gutiérrez,
  • Henrique B. Lima,
  • Gustavo M. Neves,
  • Vera L. Eifler-Lima,
  • Luisa M. da Silva,
  • Walter A. Roman Junior

摘要

Fridericia chica (Bonpl.) L.G.Lohmann, Bignoniaceae, is popularly used to treat gastrointestinal disorders. This study aimed to evaluate the antiulcer activities of a hydroalcoholic extract of F. chica and to advance the understanding of the pharmacological mechanisms through in silico, in vivo, and ex vivo studies. For the gastric healing evaluation, rats were induced with acetic acid (80%) and received the following treatments orally for 7 days, twice a day: vehicle (1 ml/kg), omeprazole (20 mg/kg), or the hydroalcoholic extract (30 mg/kg). The area of the lesion was assessed, and the healing quality was also measured by ultrasonographic analyses. Ulcer recurrence was evaluated in mice induced with interleukin (IL)-1β (1 μg/kg, i.p.). Furthermore, measurements were taken for levels of reduced glutathione (GSH) and malondialdehyde, as well as the activity of superoxide dismutase, catalase, glutathione S-transferase, N-acetyl-β-d-glycosaminidase, and myeloperoxidase. Moreover, histological and phytochemical analyses were performed. The hydroalcoholic extract accelerated gastric healing by 69.2% and prevented its recurrence by 94%. These findings were observed by histological, biochemical, and ultrasound assays, which showed mucosal preservation. Additionally, the gastric healing of the hydroalcoholic extract caused a reduction in myeloperoxidase and increased catalase activities. In addition, the reduced glutathione levels and N-acetyl-β-d-glycosaminidase activity were restored in the recurrence test. In silico, compounds from the hydroalcoholic extract, characterized by mass spectrometry (ESI-IT-MSn), revealed pathways associated with oxidative stress and inflammatory processes. The hydroalcoholic extract exhibits antiulcer effects attributed essentially to flavonoids, which demonstrate mucosal protective, anti-inflammatory, and antioxidant properties, without evidence of cytotoxicity.

Graphical Abstract