Scholarlink

<p><i>Artemisia vulgaris</i> L., Asteraceae, commonly used in traditional medicine for arthritis and gout, was previously shown to possess antioxidant and xanthine oxidase inhibitory activity. Building upon those findings, this study further investigates its active constituents and mechanisms using a multi-level pharmacological approach. The aerial parts were extracted with 96% ethanol, and the crude extract was fractionated using solvents of increasing polarity to yield hexane, ethyl acetate, and methanol fractions. Among them, the ethyl acetate fraction showed the most potent activity in suppressing reactive oxygen species and inhibiting xanthine oxidase. Six compounds were isolated from the ethyl acetate fraction and identified as kaempferol, artemisinin, luteolin-7-glucoside, 1,5-di-<i>O</i>-caffeoylquinic acid, vitexin, and diosmetin. <i>In vitro</i> xanthine oxidase inhibition assays revealed strong activity from kaempferol and artemisinin (comparable to allopurinol). Molecular docking and dynamics simulations validated their binding to xanthine oxidase. Acute oral toxicity testing at 5000 mg/kg in mice showed no signs of toxicity. These findings indicate that the ethyl acetate fraction, a bioactive fraction from the crude ethanolic extract of <i>A. vulgaris</i>, contains compounds with dual xanthine oxidase inhibitory and antioxidant actions, supporting its development as a safe anti-gout phytotherapeutic candidate.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Artemisia vulgaris Extract: A Multi-approach Study on Its Anti-gout Potential Through Xanthine Oxidase Inhibition, Antioxidant Activity, and Pharmacokinetics

  • 期刊论文

  • 发表日期：

    2025年7月28日

查看全文
  • Trinh Pham Thi Nhat,
  • Danh Tong Thanh,
  • Hang Dang Thi,
  • Tran Nguyen Ngoc Huyen,
  • Anh Nguyen Thi Hong,
  • Cuong Tran Hoai,
  • Tai Do Chiem,
  • Thanh Le Ba,
  • Hung Quach Tong,
  • Trang Nguyen Thi Thuy,
  • An Tran Nguyen Minh,
  • Dung Le Tien

摘要

Artemisia vulgaris L., Asteraceae, commonly used in traditional medicine for arthritis and gout, was previously shown to possess antioxidant and xanthine oxidase inhibitory activity. Building upon those findings, this study further investigates its active constituents and mechanisms using a multi-level pharmacological approach. The aerial parts were extracted with 96% ethanol, and the crude extract was fractionated using solvents of increasing polarity to yield hexane, ethyl acetate, and methanol fractions. Among them, the ethyl acetate fraction showed the most potent activity in suppressing reactive oxygen species and inhibiting xanthine oxidase. Six compounds were isolated from the ethyl acetate fraction and identified as kaempferol, artemisinin, luteolin-7-glucoside, 1,5-di-O-caffeoylquinic acid, vitexin, and diosmetin. In vitro xanthine oxidase inhibition assays revealed strong activity from kaempferol and artemisinin (comparable to allopurinol). Molecular docking and dynamics simulations validated their binding to xanthine oxidase. Acute oral toxicity testing at 5000 mg/kg in mice showed no signs of toxicity. These findings indicate that the ethyl acetate fraction, a bioactive fraction from the crude ethanolic extract of A. vulgaris, contains compounds with dual xanthine oxidase inhibitory and antioxidant actions, supporting its development as a safe anti-gout phytotherapeutic candidate.

Graphical Abstract