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<p>The Indian Ayurvedic formulary certified the polyherbal Ayush kwath preparation, which attracted much interest as a preventative measure against the COVID-19 pandemic. However, one of the obstacles to the Ayurvedic formulation’s acceptability is the lack of common outlines for quality control. In this study, we aimed to develop a chromatographic method to standardize the Ayush kwath. The attempt was made for simultaneous estimation of four major biomarkers, ursolic acid, cinnamic acid, zingerone, and piperine using a novel high-performance thin-layer chromatography technique. The mobile phase, petroleum ether, ethyl acetate, and acetone in the ratio of 2:3.5:0.4, v/v/v, gave a good separation, and sharp peaks at R<sub><i>f</i></sub> values (0.41 ± 0.015, 0.57 ± 0.01, 0.68 ± 0.01, and 0.77 ± 0.01) for cinnamic acid, piperine, zingerone, and ursolic acid, respectively. The linear regression analysis data for the calibration plot shows a good linear relationship with <i>R</i> ≥ 0.990 for each marker. According to the statistical analysis of the validation data, the method was precise, reproducible, and accurate for the simultaneous quantification of ursolic acid, cinnamic acid, zingerone, and piperine in the formulation. Analytical eco-scale greenness score was estimated at 0.81.</p> Graphical Abstract <p></p>

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Validation and Quantification of Key Markers in Ayush Kwath, an Ayurvedic Immunity Enhancer Polyherbal Formulation, Through High-Performance Thin-Layer Chromatography

  • 期刊论文

  • 发表日期：

    2025年1月16日

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  • Nisha Patel,
  • Kashyap Thummar,
  • Sanjay Chauhan,
  • Jigna Vadalia

摘要

The Indian Ayurvedic formulary certified the polyherbal Ayush kwath preparation, which attracted much interest as a preventative measure against the COVID-19 pandemic. However, one of the obstacles to the Ayurvedic formulation’s acceptability is the lack of common outlines for quality control. In this study, we aimed to develop a chromatographic method to standardize the Ayush kwath. The attempt was made for simultaneous estimation of four major biomarkers, ursolic acid, cinnamic acid, zingerone, and piperine using a novel high-performance thin-layer chromatography technique. The mobile phase, petroleum ether, ethyl acetate, and acetone in the ratio of 2:3.5:0.4, v/v/v, gave a good separation, and sharp peaks at Rf values (0.41 ± 0.015, 0.57 ± 0.01, 0.68 ± 0.01, and 0.77 ± 0.01) for cinnamic acid, piperine, zingerone, and ursolic acid, respectively. The linear regression analysis data for the calibration plot shows a good linear relationship with R ≥ 0.990 for each marker. According to the statistical analysis of the validation data, the method was precise, reproducible, and accurate for the simultaneous quantification of ursolic acid, cinnamic acid, zingerone, and piperine in the formulation. Analytical eco-scale greenness score was estimated at 0.81.

Graphical Abstract