Scholarlink

<p>In the present study, a novel biological process has been demonstrated in which <i>Clostridium ljungdahlii</i> DSM 13528, an acetogenic gas-fermenting organism, produces ethanol as the primary metabolic product. The process offers environmental sustainability, as the strain utilizes carbon dioxide and carbon monoxide, as the carbon source and electron donor. Modulation of the headspace gas composition (CO₂:CO ratio) and reactor headspace volume yielded a maximum biomass titer of 159.1 ± 10.8 mg/L with a productivity of 1.5 ± 0.1 mg/L/h. Sequential adaptation over 11–14 cultivation cycles resulted in a 26% increase in the specific growth rate in <i>C. ljungdahlii</i> medium with 1&#xa0;g/L yeast extract (CLY) and a 160% increase in <i>C. ljungdahlii</i> medium without yeast extract (CLNY). Subsequently, a medium engineering strategy involving modulation of metal ions and nitrogen sources (nitrate and nitrite) was implemented to redirect carbon flux from acetate towards ethanol, as the main product. These combined strategies resulted in a maximum biomass titer of 274.5 ± 26.7 mg/L and a productivity of 2.14 mg/L/h, along with a maximum ethanol titer of 18.5 ± 3.4 mmol/L and a productivity of 13.2 mg/L/h in CLY medium supplemented with tungstate (2.5 mg/L) and nitrite (18.7 mmol/L). These values are among the highest reported for <i>C. ljungdahlii</i> DSM 13528 in batch mode of cultivation. Notably, when nitrite was used as the nitrogen source, the carbon flux was predominantly redirected towards ethanol, with only trace quantities of other products detected.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Gas fermentation: process for conversion of CO and CO2 into ethanol using Clostridium ljungdahlii DSM 13,528

  • 期刊论文

  • 发表日期：

    2026年3月10日

查看全文
  • Sarvesh Kumar,
  • Gargi Goswami,
  • Debasish Das

摘要

In the present study, a novel biological process has been demonstrated in which Clostridium ljungdahlii DSM 13528, an acetogenic gas-fermenting organism, produces ethanol as the primary metabolic product. The process offers environmental sustainability, as the strain utilizes carbon dioxide and carbon monoxide, as the carbon source and electron donor. Modulation of the headspace gas composition (CO₂:CO ratio) and reactor headspace volume yielded a maximum biomass titer of 159.1 ± 10.8 mg/L with a productivity of 1.5 ± 0.1 mg/L/h. Sequential adaptation over 11–14 cultivation cycles resulted in a 26% increase in the specific growth rate in C. ljungdahlii medium with 1 g/L yeast extract (CLY) and a 160% increase in C. ljungdahlii medium without yeast extract (CLNY). Subsequently, a medium engineering strategy involving modulation of metal ions and nitrogen sources (nitrate and nitrite) was implemented to redirect carbon flux from acetate towards ethanol, as the main product. These combined strategies resulted in a maximum biomass titer of 274.5 ± 26.7 mg/L and a productivity of 2.14 mg/L/h, along with a maximum ethanol titer of 18.5 ± 3.4 mmol/L and a productivity of 13.2 mg/L/h in CLY medium supplemented with tungstate (2.5 mg/L) and nitrite (18.7 mmol/L). These values are among the highest reported for C. ljungdahlii DSM 13528 in batch mode of cultivation. Notably, when nitrite was used as the nitrogen source, the carbon flux was predominantly redirected towards ethanol, with only trace quantities of other products detected.

Graphical abstract