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<p>1,3-propanediol (1,3-PDO) is an important monomer for polyester production with broad industrial applications. <i>Klebsiella pneumoniae</i> is currently recognized as a highly efficient producer of 1,3-PDO. However, the higher cost of glycerol substrate compared with glucose limits the economic feasibility of this route. In the present study, the <i>K. pneumoniae</i> strain FMME-KP was extensively engineered for efficient 1,3-PDO production from glucose. Strategies included the introduction of a glycerol synthetic pathway from glucose, balancing carbon distribution between biomass formation and product synthesis, alleviating carbon catabolite repression, and strengthening the 1,3-PDO biosynthetic route. The optimized strain, <i>K. pneumoniae</i> GZ31, produced 84.7&#xa0;g/L of 1,3-PDO, achieving a yield of 0.51&#xa0;g/g and a productivity of 1.74&#xa0;g/L/h.</p>

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Metabolic engineering of Klebsiella pneumoniae for enhanced 1,3-propanediol production

  • 期刊论文

  • 发表日期：

    2026年1月5日

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  • Cong Gao,
  • Shaolun Zhang,
  • Guangjie Liang,
  • Kaifang Liu,
  • Jia Liu,
  • Liming Liu

摘要

1,3-propanediol (1,3-PDO) is an important monomer for polyester production with broad industrial applications. Klebsiella pneumoniae is currently recognized as a highly efficient producer of 1,3-PDO. However, the higher cost of glycerol substrate compared with glucose limits the economic feasibility of this route. In the present study, the K. pneumoniae strain FMME-KP was extensively engineered for efficient 1,3-PDO production from glucose. Strategies included the introduction of a glycerol synthetic pathway from glucose, balancing carbon distribution between biomass formation and product synthesis, alleviating carbon catabolite repression, and strengthening the 1,3-PDO biosynthetic route. The optimized strain, K. pneumoniae GZ31, produced 84.7 g/L of 1,3-PDO, achieving a yield of 0.51 g/g and a productivity of 1.74 g/L/h.