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<p>Discovery and identification of robust biodetoxification strain is crucial for the sustainable and efficient operation of lignocellulosic biorefining process. <i>Paecilomyces variotii</i> FN89, a recently isolated mesophilic filamentous fungi, was herein shown to be able to biodegrade lignocellulose-derived inhibitors including furfural (1.5&#xa0;g/L), 5-hydroxymethylfurfural (4&#xa0;g/L), acetic acid (4&#xa0;g/L), hydroxybenzaldehyde (0.2&#xa0;g/L), syringaldehyde (0.2&#xa0;g/L), and vanillin (1.5&#xa0;g/L) efficiently and completely. <i>P. variotii</i> FN89 was adapted to mixed inhibitors and relatively low dissolved oxygen conditions, which can detoxify both the highly viscosity hydrolysate and solid biomass with the well preserve of fermentable sugars and no addition of any nutrients. Two biorefinery chains involving biodetoxification process were thus established to cope with different forms of pretreated biomass for cellulosic lactic acid production. The cellulosic lactic acid titers were above 100&#xa0;g/L from 25% (w/w) solids loading pretreated wheat straw. The global transcriptome analysis of <i>P. variotii</i> FN89 in the presence of mixed inhibitors suggested that the glycolysis pathway and pentose phosphate pathway were repressed while tricarboxylic acid cycle was enhanced, ensuring the complete degradation of the inhibitors-derived intermediates and efficient energy supply. This study provided a unique and practical biodetoxification strain for lignocellulosic biorefinery, as well as enriched the knowledge of the molecular basis of lignocellulose-derived inhibitors tolerance and carbohydrates metabolism of <i>P. variotii</i>.</p>

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Harnessing Paecilomyces variotii to remove lignocellulose-derived inhibitors: potential evaluation, lignocellulosic biorefining route design, and transcriptome analysis

  • 期刊论文

  • 发表日期：

    2025年8月25日

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  • Bin Zhang,
  • Shinan Wu,
  • Agustian Chen,
  • Xia Yi,
  • Jie Bao

摘要

Discovery and identification of robust biodetoxification strain is crucial for the sustainable and efficient operation of lignocellulosic biorefining process. Paecilomyces variotii FN89, a recently isolated mesophilic filamentous fungi, was herein shown to be able to biodegrade lignocellulose-derived inhibitors including furfural (1.5 g/L), 5-hydroxymethylfurfural (4 g/L), acetic acid (4 g/L), hydroxybenzaldehyde (0.2 g/L), syringaldehyde (0.2 g/L), and vanillin (1.5 g/L) efficiently and completely. P. variotii FN89 was adapted to mixed inhibitors and relatively low dissolved oxygen conditions, which can detoxify both the highly viscosity hydrolysate and solid biomass with the well preserve of fermentable sugars and no addition of any nutrients. Two biorefinery chains involving biodetoxification process were thus established to cope with different forms of pretreated biomass for cellulosic lactic acid production. The cellulosic lactic acid titers were above 100 g/L from 25% (w/w) solids loading pretreated wheat straw. The global transcriptome analysis of P. variotii FN89 in the presence of mixed inhibitors suggested that the glycolysis pathway and pentose phosphate pathway were repressed while tricarboxylic acid cycle was enhanced, ensuring the complete degradation of the inhibitors-derived intermediates and efficient energy supply. This study provided a unique and practical biodetoxification strain for lignocellulosic biorefinery, as well as enriched the knowledge of the molecular basis of lignocellulose-derived inhibitors tolerance and carbohydrates metabolism of P. variotii.