<p>Extremophiles pose challenges to microbiologists and researchers by inhabiting the environments with extreme conditions such as alkalinity, acidity, dryness, salinity, pressure and high temperature. The research on the extremophilic microbial communities such as the thermophiles, halophiles, psychrophiles and piezophiles has increased exponentially in past couple of decades as of their ability to produce an extensive range of enzymes with stability and catalytic activity under harsh conditions. These features of extremophilic enzymes make them an amazing alternative for applications in biotechnological, textile, detergent, pharmaceutical and food industries. Adding more, these enzymes serve as cornerstone for environmentally conscious and industrial sustainability. Further, advancements in omics approaches provide new insights into discovering the diversity of unculturable microbes. The multi-omics approaches provide a means to explore the extremophilic diversity, find out novel genes, enzymes and essential products with industrial relevance. Thus, the study of the extremophiles and their enzymes with potential applications is emerging in the recent years. The present review focuses on the biodiversity of extremozymes producing microbes, omics approaches for exploring these enzymes and major applications in diverse industries.</p>