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<p>Filamentous fungi (FF) are ubiquitous and provide crucial functions in agriculture, pharmaceutical research, and both vegetative and animal nutritional support. As more and more fungal species’ genomes become publicly available, research on FF has opened up new avenues for genetic modification. The extensive use and rapid advancement of CRISPR/Cas9 technology in FF is laying the foundation for its eventual application in other contexts. When we look at the different ways that CRISPR/Cas9 technology is used in FF, we focus on how well it can change genomes by tagging genes, changing their regulation, disrupting genes, deleting genes, and other similar methods. Some of the problems with making a CRISPR framework to change FF are the Cas9 gene’s nuclear localization sequence (NLS), unintended consequences, and the need for good ways to change things. Eliminating these barriers could lead to widespread adoption of this method. This study provides a comprehensive exploration of CRISPR-Cas’s revolution in FF, including its potential improvements, ethical issues, and discoveries in its applications.</p>

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Harnessing the CRISPR/Cas9 in filamentous fungi for the production of secondary metabolites

  • 期刊论文

  • 发表日期：

    2025年3月11日

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  • Md Dilshad Karim,
  • Md Abuhena,
  • Lutfur Rahman,
  • Jubair Al rashid

摘要

Filamentous fungi (FF) are ubiquitous and provide crucial functions in agriculture, pharmaceutical research, and both vegetative and animal nutritional support. As more and more fungal species’ genomes become publicly available, research on FF has opened up new avenues for genetic modification. The extensive use and rapid advancement of CRISPR/Cas9 technology in FF is laying the foundation for its eventual application in other contexts. When we look at the different ways that CRISPR/Cas9 technology is used in FF, we focus on how well it can change genomes by tagging genes, changing their regulation, disrupting genes, deleting genes, and other similar methods. Some of the problems with making a CRISPR framework to change FF are the Cas9 gene’s nuclear localization sequence (NLS), unintended consequences, and the need for good ways to change things. Eliminating these barriers could lead to widespread adoption of this method. This study provides a comprehensive exploration of CRISPR-Cas’s revolution in FF, including its potential improvements, ethical issues, and discoveries in its applications.